| Application | Recommended Dilution |
|---|---|
| IHC | 1:20-1:200 |
| IF | 1:200-1:500 |
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA855052LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA855052LA01HU at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CSB-PA855052LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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