Immunization of rabbits with the synthesized peptide derived from PAK1/PAK2/PAK3 around the phosphorylation site of T423/402/421 to obtain the phospho-PAK1/PAK2/PAK3 (T423/402/421) polyclonal antibody. It occurs as an unconjugated IgG. It has been affinity-purified from rabbit anti-serum by affinity-chromatography using epitope-specific immunogen. Validations of this antibody in WB, IHC, and ELISA applications have been done. This phospho-PAK1/PAK2/PAK3 (T423/402/421) antibody detects endogenous levels of PAK1, PAK2, and PAK3 only when phosphorylated at T423, T402, and T421 respectively.
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