Phospho-EPS15 (Tyr849) Antibody

Code: CSB-PA190074

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Size:

100μl

US$360

Quantity:

Species Reactivity: Human,Mouse,Rat

Raised in: Rabbit

Application: ELISA,WB

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Target Background

Product Details

Uniprot NO.

P42566

Target Names

EPS15

Alternative Names

EPS15 antibody; AF1PEpidermal growth factor receptor substrate 15 antibody; Protein Eps15 antibody; Protein AF-1p antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Peptide sequence around phosphorylation site of tyrosine 849 (S-A-Y(p)-P-S) derived from Human Ephrin EPS15.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot analysis of extracts from JK cells (Lane 2), using EPS15 (Phospho-Tyr849) Antibody. The lane on the left is treated with antigen-specific peptide.

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Target Background

Function(From Uniprot)

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

Gene References into Functions

Fcho1/2Eps15/RAP-2 ternary complexes to facilitate conformational activation of AP-2 by the Fcho1/2 interdomain linker to promote AP-2 cargo engagement. PMID:27237791
An interaction of Eps15 and pS227-FIP2 at the appropriate time and location in polarizing cells is necessary for proper establishment of epithelial polarity. PMID:28228550
RAB11FIP3 combines with Eps15 homology domain 1 to promote the endocytosis recycling of phosphorylation of epithelial growth factor receptor. PMID:28215104
Together, the data indicate that a CUL3-SPOPL E3 ubiquitin ligase complex regulates endocytic trafficking and formation of multivesicular bodies by ubiquitinating and degrading EPS15 at endosomes. PMID:27008177
identify USP9X as a novel regulator of EGFR endocytosis PMID:26748853
over-expression of EPS15 is a potential favorable prognostic marker in breast cancer. PMID:26289382
MLL-EPS15 translocation and individual genomic copy number alterations in monozygotic infant twins are associated with acute lymphoblastic leukemia. PMID:22581003
Ubiquitin is sufficient for Eps15 recruitment to endosomes. PMID:25260758
analysis of the selective interaction of C-terminal Eps15 homology domain proteins with specific Asn-Pro-Phe-containing partners PMID:20106972
parkin Ubld uses differential surfaces to recruit UIM regions from the S5a proteasomal subunit compared with Eps15 involved in cell signaling. PMID:19875440
Eps15 regulates clathrin coat assembly in vivo, and interactions between Eps15 homology domains and NPF motifs are involved in clathrin-coated vesicle formation during synaptic vesicle recycling PMID:12807910
JC virus binding to human glial cells induces an intracellular signal that is critical for entry and infection by a ligand-inducible clathrin and eps15-dependent mechanism. PMID:14671106
However, GFP-EH21 (a mutant Eps15) expression caused a 50% reduction in released enveloped virions, decreased formation of satellite plaques, and delayed virus spread, indicating an important role for receptor-mediated endocytosis. PMID:15767409
Our results suggest that spartin might be involved in endocytosis, vesicle trafficking, or mitogenic activity, and that impairment in one of these processes may underlie the long axonopathy in patients with Troyer syndrome. PMID:16036216
Data show that the first ubiquitin-interacting motif of Eps15 (UIM1) interacts directly with the UBL domain of ubiquilin, whereas it does not bind to ubiquitinated proteins. PMID:16159959
SGIP1alpha plays an essential role in clathrin-mediated endocytosis by interacting with phospholipids and Eps15. PMID:17626015
These results indicate that Eps15b is an endosomally localized isoform of Eps15 that is present in the Hrs complex via direct Hrs interaction and important for the sorting function of this complex. PMID:18362181
EPS15 and EPS15R mediated internalization of activated EGF receptor to result in receptor recycling as to reinforce the proliferative response to EGF. PMID:18395097
Distinct recruitment of Eps15 via Its coiled-coil domain is required for efficient down-regulation of the met receptor tyrosine kinase. PMID:19109251

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit.; [Isoform 2]: Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with HGS on bilayered clathrin coats on endosomes.

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 3419

UNIGENE: Hs.83722

KEGG: hsa:2060

STRING: 9606.ENSP00000360798

OMIM: 600051

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