PREX2 Antibody

Code: CSB-PA741122LA01HU

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US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q70Z35

Target Names

PREX2

Alternative Names

4 antibody; 5)-dependent Rac exchanger 2 antibody; 5-trisphosphate-dependent Rac exchanger 2 protein antibody; 6230420N16Rik antibody; DEP domain-containing protein 2 antibody; DEP.2 antibody; DEPDC2 antibody; FLJ12987 antibody; P-Rex2 antibody; Phosphatidylinositol 3 antibody; phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 antibody; phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein antibody; phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 antibody; PREX2 antibody; PREX2_HUMAN antibody; PtdIns(3 antibody; PtdIns(3,4,5)-dependent Rac exchanger 2 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (1417-1592AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: PREX2 antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 183, 172, 113 kDa
Observed band size: 183, 113 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA741122LA01HU at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA741122LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.

Gene References into Functions

Study demonstrates that miR-637 inhibites melanoma cell proliferation by activation of AKT signaling pathway and induces apoptosis through regulation of Bcl-2/Bax expression via targeting P-REX2a. PMID:30213289
MiR-338-3p suppresses the TNF-alpha-induced lipogenesis in sebocytes by targeting PREX2a and down-regulating PI3K/AKT signaling. PMID:28597147
complex signaling mechanisms that involve PREX2, PI3K/AKT/PTEN and downstream epigenetic machinery to deregulate expression of key cell cycle regulators PMID:27111337
PREX2 was identified as a frequently mutated gene in human melanoma. .. mutation of PREX2 can accelerate human melanoma growth. PMID:28100393
The rapid tumor onset observed in this replication attempt, compared to the original study, makes the detection of accelerated tumor growth in PREX2 expressing NRAS(G12D) melanocytes extremely difficult. PMID:28100394
Elevated PREX2 protein expression is associated with pathogenesis of hepatocellular carcinoma. PMID:28205209
knockdown of PREX2a may effectively inhibit the malignant phenotype of glioma. PMID:26795161
The results of the present study suggested that PREX2a may act as an oncogene in osteosarcoma via the inhibition of PTEN activity and activation of PI3K signaling. PMID:26718453
findings identify PREX2 as a mediator of NRAS-mutant melanoma development that acts through the PI3K/PTEN/Akt pathway to regulate gene expression of a cell cycle regulator PMID:26884185
second messengers activate the Rac1 signal, which sets in motion a cascade whereby PAKs phosphorylate and negatively regulate PREX2 to decrease Rac1 activation. PMID:26438819
PREX2 mutants are likely selected in cancer to escape PTEN-mediated inhibition of invasion. PMID:25829446
CXCL9 is involved in the invasion ability of hepatocellular carcinoma cells possibly through up-regulation of its potential effector PREX2. PMID:25151370
Results demonstrate that miR-338-3p affects gastric cancer progression through PTEN-AKT signaling by targeting P-Rex2a in gastric cancer cells PMID:24375644
P-REX2 PH-domain-mediated inhibition of PTEN has a role in regulating insulin sensitivity and glucose homeostasis PMID:24367090
MiR-338-3p affects the PTEN/Akt pathway by down-regulating PREX2a. PMID:24140344
Analysis of whole-genome sequence data identified PREX2 (phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2)--a PTEN-interacting protein and negative regulator of PTEN in breast cancer--as a significantly mutated gene with a mutation frequency of approximately 14% in an independent extension cohort of 107 human melanomas PMID:22622578
suggests that aberrant control of PTEN by P-REX2a may represent a key tumorigenic mechanism, in agreement with recent studies supporting the pathological relevance of several other proposed PTEN regulators PMID:19861688
P-REX2 may serve as a novel link between Rac activation and the PI-3 kinase pathway. PMID:15304342
P-Rex2 is a 183 kDa protein that activates the small GTPase Rac and is regulated by phosphatidylinositol (3,4,5)-trisphosphate. PMID:15304343
the pleckstrin homology domain of the Dbl family guanine nucleotide exchange factor P-Rex2 has a role in substrate specificity and recognition PMID:15897194
identified P-REX2a as a PTEN-interacting protein; P-REX2a inhibited PTEN lipid phosphatase activity & stimulated the PI3K pathway only in the presence of PTEN; P-REX2a is a component of the PI3K pathway that can antagonize PTEN in cancer cells PMID:19729658

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Tissue Specificity

Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart.

Database Links

HGNC: 22950

UNIGENE: Hs.169943

KEGG: hsa:80243

STRING: 9606.ENSP00000288368

OMIM: 612139

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