ODR4 Antibody

CSB-PA725554LA01HU

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US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q5SWX8

Target Names

ODR4

Alternative Names

ODR4; C1orf27; TTG1; TTG1A; Protein odr-4 homolog; hODR-4; LAG1-interacting protein; Transactivated by transforming growth factor beta protein 1

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Protein odr-4 homolog protein (153-251AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate
All lanes: ODR4 antibody at 4.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 49, 38, 48 kDa
Observed band size: 52 kDa
IHC image of CSB-PA725554LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-PA725554LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Description

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Target Background

Function

May play a role in the trafficking of a subset of G-protein coupled receptors.

Gene References into Functions

LMO2, TAL1, Ttg-1, and SIL support levels of V(D)J recombination above background levels in cell culture and are also cleaved by the RAG proteins, while Hox11 and SCL are nicked but not cleaved efficiently in vitro PMID:18187418

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Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

ODR-4 family

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 24299

UNIGENE: Hs.371210

KEGG: hsa:54953

STRING: 9606.ENSP00000287859