NPFFR2 Antibody

CSB-PA015983NA01HU

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20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

Q9Y5X5

Target Names

NPFFR2

Alternative Names

NPFFR2; GPR74; NPFF2; NPGPR; Neuropeptide FF receptor 2; G-protein coupled receptor 74; G-protein coupled receptor HLWAR77; Neuropeptide G-protein coupled receptor

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence from Human Neuropeptide FF receptor 2 protein (23-43AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-PA015983NA01HU diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Description

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Target Background

Function

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Gene References into Functions

Effects of systematic N-terminus deletions and benzoylations of endogenous RF-amide peptides on NPFF1R, NPFF2R, GPR10, GPR54 and GPR103. PMID:26211894
The NPFF2 neuropeptide FF receptor phosphorylation sites have been mapped and their role identified in receptor regulation. PMID:25326382
These data show that NPFF-induced heterologous desensitization of MOP receptor signaling is mediated by GRK2 and could involve transphosphorylation within the heteromeric receptor complex. PMID:22375000
Four genetic loci were strongly and independently associated with obesity, NPY2R, NPFFR2, MC4R, and FTO. PMID:21818152
Besides regulation of reproduction, kisspeptins have a potential to mediate physiological effects on autonomic regulation and nociception in humans via the NPFF2R pathways. PMID:20600636
species-related differences in the binding properties of NPFF(2) receptors PMID:19944730
Evidence does not support the hypothesis that NPGPR is involved in feeding regulation. PMID:12609745
The BIBP3226 and PFR(Tic)amide, on Human neuropeptide FF receptor subtype 2 (hNPFF2) were investigated. PMID:15127947
neuropeptide FF analogs show that NPFF2 receptors couple to the four subunits assayed. PMID:15608144
expression of hNPFF2 receptor mRNA was up-regulated by neuropeptide FF PMID:16336216
NPFF system may also have species-specific features since the NPFF2 receptor mRNA expression differs from that reported for rat PMID:17157836
findings suggest that a common haplotype in the GPR74 gene protects against obesity, which, in part, is caused by a relief of inhibition of lipid mobilization from adipose tissue PMID:17503329
we identified hFF2 positive neurons in the forebrain and medulla oblongata of individuals who died suddenly. PMID:18565622

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in

Database Links

HGNC: 4525

UNIGENE: Hs.99231

KEGG: hsa:10886

STRING: 9606.ENSP00000307822

OMIM: 607449

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