NOP58 Antibody

Code: CSB-PA896703LA01HU

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20μg50μg100μg

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IF

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Target Background

Product Details

Uniprot NO.

Q9Y2X3

Target Names

NOP58

Alternative Names

HSPC120 antibody; NOL5 antibody; NOP5 antibody; NOP5/NOP58 antibody; NOP58 antibody; NOP58 ribonucleoprotein homolog (yeast) antibody; NOP58 ribonucleoprotein homolog antibody; NOP58_HUMAN antibody; Nucleolar protein 5 antibody; Nucleolar protein 58 antibody; Nucleolar protein NOP5/NOP58 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Nucleolar protein 58 protein (1-529AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescent analysis of Hela cells using CSB-PA896703LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western Blot
Positive WB detected in: 293 whole cell lysate, 293T whole cell lysate
All lanes: NOP58 antibody at 3.75μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A375 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate, HL60 whole cell lysate
All lanes: NOP58 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

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Target Background

Function(From Uniprot)

Required for 60S ribosomal subunit biogenesis. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs.

Gene References into Functions

SUMOylation is essential for high-affinity Nop58 binding to small nucleolar riboproteins. PMID:20797632
Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments. PMID:12032086

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Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm.

Protein Families

NOP5/NOP56 family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 29926

UNIGENE: Hs.471104

KEGG: hsa:51602

STRING: 9606.ENSP00000264279