NELFCD Antibody

Code: CSB-PA815573ESR2HU

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q8IXH7

Target Names

NELFCD

Alternative Names

HSPC130 antibody; Negative elongation factor C/D antibody; NELF C antibody; NELF D antibody; NELF-C/D antibody; NELFC antibody; NELFD antibody; NELFD_HUMAN antibody; TH1 antibody; TH1-like protein antibody; TH1L antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Negative elongation factor C/D protein (346-590AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:200-1:1000
IHC	1:20-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: NELFCD antibody at 8μg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 67, 23, 66 kDa
Observed band size: 67 kDa
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA815573ESR2HU at dilution of 1:100

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Target Background

Function(From Uniprot)

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

Gene References into Functions

Trihydrophobin 1 phosphorylation by c-Src regulates MAPK/ERK signaling and cell migration. PMID:22238675
TH1 might play an important role in regulation of proliferation and invasion in human breast cancer. PMID:20735431
These results indicate that TH1 is a novel regulator to control the duration and magnitude of androgen signal transduction and might be directly involved in androgen-related developmental, physiological, and pathological processes. PMID:20069563
diverse transcriptional consequence of NELF-mediated RNAPII pausing in the human genome PMID:20028984
In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf; this specific interaction may have played a critical role in the activation of A-Raf. PMID:11952167
Trihydrophobin 1 negatively regulates A-Raf kinase PMID:14684750
A study was done of ubiquitin-dependent proeolysis of TH1L protein by E6-AP. PMID:17131388
Maternal serum Th1 cytokines concentrations increase in preterm and term delivery. PMID:17621989
TH1 interacts with PAK1 and specifically restricts the activation of MAPK modules through the upstream region of the MAPK pathway, thereby influencing cell migration. PMID:19136554