NCK2 Antibody

Code: CSB-PA015531HA01HU

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US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

O43639

Target Names

NCK2

Alternative Names

Cytoplasmic protein NCK2 antibody; GRB4 antibody; Growth factor receptor bound protein 4 antibody; NCK adaptor protein 2 antibody; Nck-2 antibody; NCK2 antibody; NCK2_HUMAN antibody; NCKbeta antibody; Non-catalytic region of tyrosine kinase adaptor protein 2 antibody; Noncatalytic region of tyrosine kinase beta antibody; SH2/SH3 adaptor protein NCK-beta antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Cytoplasmic protein NCK2 protein (1-380AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: NCK2 antibody at 8µg/ml
Lane 1: Mouse brain tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA015531HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA015531HA01HU at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA015531HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Gene References into Functions

This study provides evidence of a possible role of NCK2 as biomarker of ovarian cancer progression. PMID:29218693
There was no difference in the Nck2 mRNA expression between the SLE patients and the healthy subjects. PMID:29911835
Nck1 and Nck2 Interact with WTIP. Nck1/2 integrates nephrin with the Hippo kinase cascade through association with the adaptor protein WTIP. PMID:27033705
Data suggest that PINCH1 and Nck2 critically participate in the regulation of cellular radiosensitivity and EGFR function and downstream signaling in a cellular model of human squamous cell carcinoma. PMID:26004008
Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP). PMID:25482634
Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. PMID:24287595
NCK2 is involved in the susceptibility to opiates addiction. PMID:23533358
The hNck2 SH3 domain also exhibited pH dependent monomer-dimer transition. PMID:23524290
Data show that both HK2 and NCK2 are expressed in the retinal ganglion cell layer. PMID:23349798
Nck2 effectively influences human melanoma phenotype progression. PMID:21992144
p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. PMID:21949127
Nck2 is an adaptor protein composed of 3N-SH3 domains followed by a unique Cterminal SH2 domain. It interacts with PINCH in integrin signal transduction, cell migratio and survival. Review. PMID:21880263
biophysical analysis of Nck2 SH3 domain PMID:19956763
Nck-2 interacts with focal adhesion kinase and modulates cell motility. Using a mutational strategy, the formation of the Nck-2-FAK complex is mediated by interactions involving multiple SH2 and SH3 domains of Nck-2. PMID:11950595
Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. PMID:12110186
analysis of binding between the Tyr-phosphorylated human ephrinB2 and Nck2 SH2 domain PMID:15764601
Molecular model of the first SRC homology 3 domain of Nck2. PMID:16604428
Nck1 (Nckalpha) and Nck2 (Nckbeta and Grb4): binding specificities of both SH2 domains are essentially indistinguishable PMID:16636066
determined the NMR structures and dynamic properties of the hNck2 SH3 domains and to define their ligand binding preferences with nine proline-rich peptides derived from Wire, CAP-1, CAP-2, Prk, Wrch1, Wrch2, and Nogo PMID:16752908
Data show that Nck (isoforms 1 and 2) as a component of the CReP/PP1c holophosphatase complex contributes to maintain eIF2alpha in a hypophosphorylated state, and modulates translation and eIF2alpha signaling in response to ER stress. PMID:16835242
Demonstrate connection between septins/SOCS7/NCK signaling and the DNA damage response. PMID:17803907
the adaptors Nck and ShcA influenced adherence of S. Typhimurium to non-phagocytic cells. PMID:17906149
Data show that Nck forms a complex with an atypical PxxDY motif of the CD3epsilon tail, which encompasses Tyr166 within the activation motif and a T-cell receptor endocytosis signal. PMID:18555270
Results point to NCK2 as a disease candidate gene and further supports the GLC1B locus as an important genomic region that is associated with the genetic predisposition to glaucoma. PMID:18723748
independent roles and mechanisms of action of Nckalpha and Nckbeta in dermal fibroblast migration, which is critical for wound healing. PMID:19242519