| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:5000 |
| IHC | 1:20-1:200 |
| IF | 1:10-1:100 |
Western blot
All lanes: LRTM1 antibody at 0.4μg/ml + K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39, 30 kDa
Observed band size: 39 kDa
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA884513LA01HU at dilution of 1:100
Immunofluorescence staining of Hela cells with CSB-PA884513LA01HU at 1:13, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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