LRP8 Antibody

Code: CSB-PA623900LA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q14114

Target Names

LRP8

Alternative Names

APOER2 antibody; Apolipoprotein E receptor 2 antibody; low density lipoprotein receptor-related protein 8 antibody; Low-density lipoprotein receptor-related protein 8 antibody; LRP-8 antibody; LRP8 antibody; LRP8_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Low-density lipoprotein receptor-related protein 8 protein (131-332AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, A549 whole cell lysate, U87 whole cell lysate
All lanes: LRP8 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 106, 78, 100, 88 kDa
Observed band size: 106 kDa
Immunofluorescent analysis of HepG2 cells using CSB-PA623900LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Description

CUSABIO produces the product (CSB-PA623900LA01HU) by immunizing rabbits with the peptide corresponding to amino acids 131-332 of the recombinant human LRP8. The anti-LRP8 antibody is eventually purified from rabbit antiserum by protein G affinity chromatography. Its purity is greater than 95%. It occurs as an unconjugated IgG. And it only reacts with human LRP8 protein. This LRP8 polyclonal antibody is suitable for the detection of LRP8 protein in ELISA, WB, and IF applications.

The LRP8 protein, also known as apolipoprotein E receptor 2 (APOER2), regulates the uptake and clearance of lipoproteins and mediates signaling events in response to extracellular ligands, with important implications for brain development and function, as well as for metabolic disorders and neurodegenerative diseases.

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Target Background

Function(From Uniprot)

Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2. Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells.

Gene References into Functions

ABGL4, LRP8 and PCSK9 polymorphisms and gene interactions increase cardiometabolic risk. PMID:27853278
This study elucidates that the contracted-open conformation of ligand-bound ApoER2 at neutral pH resembles the contracted-closed conformation of ligand-unbound low-density lipoprotein receptor at acidic pH in a manner suggestive of being primed for ligand release even prior to internalization. PMID:28446613
LRP8 is a risk gene for psychosis. PMID:26637325
Results show that none of the SNPs were significantly associated with myocardial infarction (MI). Interestingly, haplotype based association analysis showed TG and CG of rs10788952 and rs7546246 significantly associated with MI and in particular, haplotype TG was positively correlated with the risk of MI, as this increased the LDL and total cholesterol level in MI patients in south Indians. PMID:29032149
These results reveal an association between ApoER2 isoform expression and Alzheimer's disease. PMID:26902204
ApoER2 contributes cooperatively with endothelial cell protein C receptor and protease activated receptor 1 to APC-initiated endothelial antiapoptotic and barrier protective signaling. PMID:26800564
two SNPs (rs3737984 and rs2297660) in ApoER2 gene had significant association with dyslipidemia in Thai ethnic PMID:26817215
A novel TACGC risk haplotype in the LRP8 gene that is present in patients with CAD and MI but not in normal controls. PMID:24867879
results of this study demonstrated the presence of reelin, its receptors VLDLR and ApoER2 as well as Dab1 in the ENS and might indicate a novel role of the reelin system in regulating neuronal plasticity and pre-synaptic functions in the ENS. PMID:24844606
Data suggest that PS1/gamma-secretase-dependent processing of the reelin receptor ApoER2 inhibits reelin expression and may regulate its signaling. PMID:24344333
By incorporating the information from bioinformatics and RNA expression analyses, we identified at least two of the most promising risk genes for alcohol dependence: APOER2 and UBAP2 PMID:23739027
TCCGC haplotype at the 3'-terminal block of the LRP8 gene confers a protective role in the development of familial and early-onset coronary artery disease and/or myocardial infarction. PMID:23524007
In a Chinese unrelated Han population, variants within the LRP8 gene do not convey the risk of developing Parkinson's disease. PMID:22889673
results identify LRP8 as a novel positive factor of canonical Wnt signaling pathway PMID:22589174
genetic variant R952Q of LRP8 is associated with increased plasma TG levels in patients who are overweight and have premature CAD/MI and history of smoking. PMID:22404453
ApoEr2 plays important roles in structure and function of CNS synapses and dendritic spines PMID:21347244
Deficient sLRP-amyloid-beta binding might precede and correlate later in disease with an increase in the tau/Abeta42 CSF ratio and global cognitive decline in mild cognitive impairment individuals converting into Alzheimer's disease. PMID:21157031
LRP8 gene polymorphisms influence plasma cholesterol levels as well as size and composition of LDL particles PMID:21316997
the expression of ApoER2 may serve as a trait marker for major depressive disorder. PMID:20493228
There were seven polymorphisms in apoE receptor 2 in Japanese sporadic Alzheimer disease patients, but no association of these polymorphisms with Alzheimers. PMID:20208369
Differential functions of ApoER2 and very low density lipoprotein receptor in Reelin signaling depend on differential sorting of the receptors. PMID:19948739
ApoEr2 regulates cell movement, and both X11alpha and Reelin enhance this effect. PMID:19720620
transmembrane domain and PXXP motif excludes it from carrying out clathrin-mediated endocytosis PMID:12621059
Data show that the apolipoprotein E receptor 2 binding domain of apolipoprotein E is in the 1-165 amino terminal region, whereas the carboxy terminal 230-299 region of apoE is required for efficient initial association with phospholipids. PMID:12950167
Complete molecular structure examined by nuclear magnetic resonance. PMID:16034672
ApoEr2 can form a multiprotein complex with NMDA receptor subunits and PSD95 PMID:16332682
Reelin signals by binding to two transmembrane receptors, apolipoprotein E receptor 2 (Apoer2) and very-low-density lipoprotein receptor. PMID:16481437
In conclusion, results from the two independent samples of black women provide consistent evidence that SNP rs2297660 in LRP8 is associated with fetal growth. PMID:16642433
The effect of Dab1 on APP and apoEr2 processing in transfected cells and primary neurons is reported. PMID:16951405
The presence of three splice variants of ApoER2 on platelets was confirmed by immuno-blotting, with ApoER2Delta4-5 being the most abundantly expressed splice variant. PMID:17470198
However, this polymorphism increased the risk of AD conferred by the MAPK8IP1 G allele. PMID:17614163
A nonconservative substitution, R952Q, in LRP8 was significantly associated with susceptibility to premature CAD and/or MI by use of both population-based and family-based designs. PMID:17847002
Fyn, due in part to its effects on Dab1, regulates the phosphorylation, trafficking, and processing of APP and apoEr2. PMID:18089558
protein C and APC may directly promote cell signaling in other cells by binding to ApoER2 and/or GPIbalpha PMID:18489431
Study showed no evidence for association of genetic variants in the LRP8 gene with familial and sporadic myocardial infarction. PMID:18592168
ligation of ApoER2 by APC signals via Dab1 phosphorylation and subsequent activation of PI3K and Akt and inactivation of GSK3beta, thereby contributing to APC's beneficial effects on cells. PMID:19116273
Our data suggest that LRP8 R952Q variant may have an additive effect to APOE epsilon2/epsilon3/epsilon4 genotype in determining ApoE concentrations and risk of MI in an Italian population PMID:19439088
apolipoprotein E receptor 2 (ApoER2, LRP8), a member of the LDL receptor family, is a platelet receptor for FXI. PMID:19661487
Sequential proteolytic processing of murine ApoER2 results in the release of its intracellular domain by the protease gamma-secretase. The prior cleavage of its extracellular domain is determined by the glycosylation state of the receptor. PMID:12871934