LAMA4 Antibody

Code: CSB-PA621948LA01HU

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20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IF

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Target Background

Product Details

Uniprot NO.

Q16363

Target Names

LAMA4

Alternative Names

CMD1JJ antibody; DKFZp686D23145 antibody; LAMA3 antibody; LAMA3, formerly antibody; LAMA4 1 antibody; LAMA4 antibody; LAMA4*-1 antibody; LAMA4_HUMAN antibody; Laminin alpha 4 antibody; Laminin alpha 4 chain antibody; Laminin subunit alpha 4 precursor antibody; Laminin subunit alpha-4 antibody; Laminin-14 subunit alpha antibody; Laminin-8 subunit alpha antibody; Laminin-9 subunit alpha antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Laminin subunit alpha-4 protein (1593-1823AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate
All lanes: LAMA4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 203, 202, 13 kDa
Observed band size: 203 kDa
Immunofluorescence staining of U251 cells with CSB-PA621948LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Target Background

Function(From Uniprot)

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Gene References into Functions

High LAMA4 expression is associated with gastric cancer. PMID:30015861
Disruption of LAMA4, LAMA5, and LAMB2 or the laminin-receptor interaction occurs in neuromuscular diseases including Pierson syndrome and Lambert-Eaton myasthenic syndrome (LEMS). (Review) PMID:27614294
highlight MCAM and LAMA4 as prime candidates for RCC prognosis and therapeutic targeting PMID:26921326
LAMA4-integrin signalling affects chondrocyte morphology and gene expression, contributing to cluster formation in osteoarthritic chondrocytes. PMID:26295200
LAMA4 expression is lowered in preeclamptic placentas and promotes trophoblast cell invasion, migration, and angiogenesis. hypoxia-reoxygenation decreases LAMA4 expression and decouple the relationship between LAMA4 expression and p38 and ERK activation. PMID:26059342
Oxidative stress plays a vital role in controlling expression of LAMA4 through MAPK signaling pathways, which suggests a possible pathological mechanism of pre-eclampsia. PMID:25676580
Laminins 411 and 421 differentially promote tumor cell migration via alpha6beta1 integrin and MCAM (CD146). PMID:24951930
Data from experiment with peptide fragments suggest laminin alpha4 (and laminin alpha5) may be part of host defense response and may protect tissues from invading pathogens. PMID:20433883
Laminins with alpha1, alpha4, and alpha5 chains are compared to determine laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. PMID:12393739
Data report the identification of a splice variant of the human laminin alpha4 subunit transcript containing 21 extra nucleotides. PMID:12445830
The G domain of laminin alpha4 chain is a specific, high affinity ligand for alphavbeta3 & alpha3beta1 integrin heterodimers. These integrins, with alpha6beta1, cooperatively mediate endothelial cell-alpha4 laminin interaction & blood vessel development. PMID:12454288
The alpha4 laminin subunit regulates vascular endothelium cell survival. PMID:14980521
laminin alpha4 LG4 module may play an important role in cell adhesion and/or vessel wall formation in the skin by interacting with syndecan-2 and/or -4. PMID:15086543
Downregulation of laminin alpha4 chain expression inhibits glioma invasion PMID:15915502
laminin isoform changes are associated with brain tumor invasion and angiogenesis [review] PMID:16146715
This suggests that G(1121-1139) peptide-containing proteins may perform their biological functions by interacting with alphavbeta3 integrin. PMID:16824487
alpha4 chain Lms have a de-adhesive function and could thus play a role in detachment, migration and invasion of renal carcinoma cells in vivo. PMID:17533363
2 novel mutations (2828C>T [Pro943Leu] & 3217C>T [Arg1073X]) in the integrin-interacting domain of the LAMA4 gene reduce its integrin binding, & cause endothelial cell loss and heart failure. PMID:17646580
LAMA4 is specifically up-regulated on both mRNA and protein levels in hepatocelluar carcinoma; potential role in hepatocarcinogenesis and tumor progression. PMID:18084776
Concomitant changes take place in laminin- and collagen-binding receptors. Laminin-411 reduces adhesion to laminin-511 and fibronectin, suggesting that tumor cells could utilize laminin-411 in their invasive behavior PMID:18496706
LM alpha4 and beta2 have roles in in vitro migration and in vivo tumorigenicity of prostate cancer cells PMID:19048114

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Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.

Tissue Specificity

In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn t

Database Links

HGNC: 6484

UNIGENE: Hs.654572

KEGG: hsa:3910

STRING: 9606.ENSP00000230538

OMIM: 600133

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