JAM3 Antibody

Code: CSB-PA874828LA01HU

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US$83

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Species Reactivity: Human, Rat

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q9BX67

Target Names

JAM3

Alternative Names

FLJ14529 antibody; JAM 2 antibody; JAM 3 antibody; JAM C antibody; JAM-2 antibody; JAM-3 antibody; JAM-C antibody; JAM2 antibody; Jam3 antibody; JAM3_HUMAN antibody; JAMC antibody; Junctional adhesion molecule 3 antibody; Junctional adhesion molecule 3 precursor antibody; Junctional adhesion molecule C antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Junctional adhesion molecule C protein (119-235AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Rat heart tissue
All lanes: JAM3 antibody at 2.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 30 kDa
Observed band size: 45 kDa
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA874828LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA874828LA01HU at dilution of 1:100
Immunofluorescence staining of Hela cells with CSB-PA874828LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Target Background

Function(From Uniprot)

Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium. Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation. Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). Plays a role in angiogenesis. Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion.; Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.

Gene References into Functions

JAM-C plays an important role in maintaining VEGR2 expression to promote retinal pigment epithelial cell survival under oxidative stress. PMID:28203682
JAM-C controls Src family kinase (SFK) activation in leukemic stem cells and leukemia-initiating cell activity with exacerbated SFK activation was uniquely found within the JAM-C-expressing leukemic stem cell compartment. PMID:28972073
The role of JAM-C in the engraftment of human lymphoma B cells in mice was investigated. The binding of anti-JAM-C antibodies inhibited the phosphorylation of ERK1/2, without affecting other signaling pathways, identifying for the first time the intracellular MAPK cascade as the JAM-C-driven signaling pathway in JAM-C(+) B cells. PMID:27256571
blocking JAM-C can reduce the number of atherogenic monocytes/macrophages in plaques by emigration. PMID:27442505
Palmitoylation of JAM-C promotes its localization to tight junctions and inhibits transwell migration of A549 lung cancer cells. PMID:28196865
Study provides evidence that JAM-C downregulation may contribute to acute pancreatitis-associated lung injury via reverse transendothelial migration of neutrophils. PMID:26841848
Suggest JAM3-M4 methylation as a biomarker for diagnosis of preneoplastic and neoplastic lesions of the cervix. PMID:26517242
JAM-C inactivation in endothelial cells resulted in increased spreading on fibronectin and enhanced sprouting in vitro in a manner dependent on beta1-integrin and on the activation of the small GTPase RAP1. PMID:26311310
indicate that JAM-C may be a therapeutic target for preventing and treating lymphatic metastases PMID:24584816
These findings provide evidence for a role for endothelial cell JAM-C in tumor growth and aggressiveness as well as recruitment of pericytes to newly formed blood vessels in a model of ovarian cancer. PMID:23825230
Our study confirms the importance of JAM3 as a component of the junctional complexes and its deficiency leading to a distinctive and catastrophic neonatal presentation of cataracts and hemorrhagic destruction of the brain. PMID:23255084
In the present study, we investigated the role of JAM-C in homing of human B cells, using a xenogeneic nonobese diabetic/severe combined immunodeficient mouse model. PMID:23221386
JAM-C expression was identified in human and murine melanoma cell lines, in human malignant melanoma, as well as in metastatic melanoma including melanoma lung metastasis PMID:21593193
Our results suggest that JAM3 is essential for maintaining the integrity of the cerebrovascular endothelium as well as for normal lens development in humans PMID:21109224
Soluble JAM-C mediates facets of angiogenesis in vitro and contributes the angiogenic potential of rheumatoid arthritis synovial fluid. PMID:20592283
Letter: investigated platelet JAM-C following platelet activation by several agonists. PMID:20135068
molecular cloning & role as VE-JAM/JAM 2 receptor PMID:11823489
JAM3 is a strong candidate gene for hypoplastic left heart syndrome PMID:11944976
Facilitates JAM2 interaction with alpha4beta1 PMID:12070135
The junctional adhesion molecule 3 (JAM-3) on human platelets is a counterreceptor for the leukocyte integrin Mac-1. PMID:12208882
JAM-C(3) MAY play a role in desmosomal structure/function. PMID:15194813
JAM-C participates in neutrophil transmigration and thereby provides a novel molecular target for antagonizing interactions between vascular cells that promote inflammatory vascular pathologies PMID:15485832
Junctional adhesion molecule-C participates in the later steps of the leukoendothelial adhesion and transmigration cascade in an acute pulmonary inflammation model using transgenic mouse tissue overexpressing JAM-C. PMID:15879142
the homophilic interaction of JAM-C can mediate tumor cell-endothelial cell interactions and may have a role in tumor cell metastasis PMID:16118203
JAM-C is up-regulated by oxLDL and may thereby contribute to increased inflammatory cell recruitment during atherosclerosis PMID:16195363
JAM-C is an integral component of the dermal fibroblast adherens-like junction, and co-localizes with ZO-1. PMID:16916751
Helps to regulate vascular permeability and pathologic angiogenesis through modulation of vascular endothelium (VE) contractility and VE-cadherin-mediated adhesion. PMID:17116731
the expression of JAM-C promotes metastasis by enhancing both the adhesion of cancer cells to extracellular matrices and the subsequent invasion PMID:17227766
expression of JAM-C in different B-cell lymphomas reveals a disease-specific pattern and allows a clear distinction between JAM-C-lymphoproliferative syndromes and JAM-C+ ones PMID:17429428
The results of these studies suggest a role for JAM-C in the pathogenesis of arthritis. PMID:17612407
blockade of JAM-B/-C interaction reduced monocyte numbers in the extravascular compartment through increased reverse transmigration rather than by reduced transmigration PMID:17625065
Examine JAM-3 expression in normal/inflammed lymphatic endothelium. PMID:17822725
study shows JAM-C was expressed in human sural nerves with an expression profile similar to that seen in mice PMID:18048693
JAM-C *junctional adhesion molecule C) mediates recruiting and retaining leukocytes in the Rheumatoid arthritis synovium PMID:18821692
JAM-C expression is variable in different epithelial cell lines with co-localization at tight junctions. PMID:19143587
JAM-C gene expression is transcriptionally induced during activation of T lymphocytes via a calcineurin-dependent signaling pathway. PMID:19204148
The regulation of EC junctional integrity involves the coordinated and dynamic modification of localization and activity of junctional stabilizers such as the integrin beta(3) and the destabilizer, JAM-C. PMID:19461049
These studies suggest a function for coxsackievirus and adenovirus receptor (CAR) in male fertility. PMID:16410001
Functions as a facilitator for JAM2/alpha4beta1 interactions PMID:12070135
Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor. PMID:11590146

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell junction. Cell junction, desmosome. Cell junction, tight junction.; [Soluble form of JAM-C]: Secreted.

Protein Families

Immunoglobulin superfamily

Tissue Specificity

Detected on round and elongated spermatids (at protein level). Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (

Database Links

HGNC: 15532

UNIGENE: Hs.150718

KEGG: hsa:83700

OMIM: 606871

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