| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:5000 |
| IP | 1:200-1:2000 |
Western blot
All lanes: IFNA21 antibody at 2µg/ml
Lane 1: NIH/3T3 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunoprecipitating IFNA21 in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA011039EA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA011039EA01HU (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)
Email: support@cusabio.com
Distributors Worldwide