HSPA6 Antibody

Code: CSB-PA863082HA01HU

Rate （5.0）
Review （1）

Size:

20μg50μg100μg

US$83

Quantity:

Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P17066

Target Names

HSPA6

Alternative Names

Heat shock 70 kDa protein 6 antibody; Heat shock 70 kDa protein B' antibody; Heat shock 70 kDa protein B'' antibody; heat shock 70kD protein 6 (HSP70B') antibody; Heat shock 70kDa protein 6 antibody; HSP70B' antibody; HSP70B-Prime antibody; HSP76_HUMAN antibody; HSPA6 antibody; OTTHUMP00000032372 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Heat shock 70 kDa protein 6 protein (1-637AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: HSPA6 antibody at 2µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Applications : IP

Sample type: Human Human neuroblastoma cell

Review: The cells were incubated with cortisol (1 μM) for 60 min. GR was co-immunoprecipitated with anti-Hsp70 and -Hsp90 antibodies (the left side). Expression of Hsp70, Hsp90, GR, and β-actin in total cell lysates is shown in the right side.

By Anonymous

Target Background

Function(From Uniprot)

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.

Gene References into Functions

during recovery from neuronal stress, HSPA6 localized with perispeckles that have been characterized as transcription sites; the stress-induced association of HSPA6 with perispeckles displayed the greatest dynamism compared to the interaction of HSPA6 or HSPA1A with other stress-sensitive cytoplasmic and nuclear structures; this suggests involvement of HSPA6 in recovery of neurons from cellular stress PMID:27527722
In vivo efficacy experiments with HSPA6 siRNA and MFH were performed using the A2780cp20 and HeyA8 ovarian cancer mouse models. A significantly reduction in tumor growth rate was observed with combination therapy. PES and MFH efficacy were also evaluated in the HeyA8 intraperitoneal tumor model, and resulted in robust antitumor effects. PMID:28223424
measurable HSP70B' was not associated with graft versus host disease following allogeneic hematopoietic cell transplantation PMID:27020764
endothelial nitric oxide synthase induces heat shock protein HSPA6 (HSP70B') in human arterial smooth muscle cells PMID:26656590
Data indicate that heat shock protein 90kD(HSP90) inhibition induces heat shock 70kD protein 6 (HSP70B') expression. PMID:25957766
HSPA6 regulation by TNIP1 occurs in promoter regions lacking binding sites for known TNIP1-repressed transcription factors PMID:25447897
Hsp70B' also formed complexes with Hsp40 suggesting a common co-chaperone for HSP70 family members. PMID:20084477
Heat shock protein 70B' (HSP70B') expression and release in response to human oxidized low density lipoprotein immune complexes in macrophages. PMID:20348092
These findings are likely to be important in pathological conditions in which Hsp70B' contributes to cell survival. PMID:18229458
Hsp70B' expressed on colon cells after proteasome inhibition was most closely related to Hsp72. They shared 100% AA identity in the peptide-binding region but differed in the lid and C-terminal domains.Hsp70B' appeared to have diverged recently. PMID:18347947