| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:5000 |
| IF | 1:50-1:200 |
Western blot
All lanes: HLA-DRB1 antibody at 2µg/ml
Lane 1: A375 whole cell lysate
Lane 2: Raji whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Immunofluorescent analysis of A375 cells using CSB-PA19179A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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