HIST1H1E (Ab-25) Antibody

CSB-PA010380PA25nme1HU

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Review （1）

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20μl50μl100μl

US$88

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF, ChIP

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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P10412

Target Names

HIST1H1E

Alternative Names

H1 histone family member 4 antibody; H1.4 antibody; H14_HUMAN antibody; H1E antibody; H1F4 antibody; Hist1h1e antibody; Histone 1 H1e antibody; Histone cluster 1 H1e antibody; Histone H1 antibody; Histone H1.4 antibody; Histone H1B antibody; MGC116819 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence around site of Lys (25) derived from Human Histone H1.4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC, IF, ChIP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Native Chromatin Immunoprecipitation(ChIP) Protocol

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
All lanes: HIST1H1E antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Description

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Sample type: Human embryonic stem cell lines and HEK293T cells

Sample dilution: IHC: 1:200, WB: 1:1000

Review: describe

By Anonymous

Target Background

Function

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

Gene References into Functions

Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. PMID:26209608
This study identified and confirmed HIST1H1E protein changes within the postsynaptic density in schizophrenia. PMID:25048004
the N-terminal domain of H1 is an important determinant of affinity and specificity of H1-chromatin interactions. PMID:22425985
PKA-mediated H1.4S35 phosphorylation dissociates H1.4 from mitotic chromatin but also suggest that this phosphorylation is necessary for specific mitotic functions. PMID:21852232
Allele-specific underacetylation of histone H4 downstream from promoter is associated with X-inactivation in human cells. PMID:12498347
the lysine residue adjacent to the phosphorylation site found on the serine residue on the H1.4 peptide KARKSAGAAKR was also shown to be methylated, raising the question of whether the hypothesized "methyl/phos" switch could be extended to linker histones PMID:15595731
Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins PMID:19144645