HIST1H1B (Ab-188) Antibody

Code: CSB-PA010377PA188nphHU

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20μl50μl100μl

US$88

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

P16401

Target Names

HIST1H1B

Alternative Names

H1 antibody; H1 histone family member 5 antibody; H1.5 antibody; H15 HUMAN antibody; H15_HUMAN antibody; H1B antibody; H1F5 antibody; H1s 3 antibody; Hist1h1b antibody; Histone 1 H1b antibody; Histone cluster 1 H1b antibody; Histone H1.5 antibody; Histone H1a antibody; Histone H1b antibody; Histone H1s 3 antibody; MGC126630 antibody; MGC126632 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence around site of Ser (188) derived from Human Histone H1.5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:100-1:1000
IHC	1:20-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate
All lanes: HIST1H1B antibody at 1.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010377PA188nphHU at dilution of 1:100

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Target Background

Function(From Uniprot)

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

Gene References into Functions

Data indicate that normal ovarian tissues strongly expressed histone H1.5, whereas ovarian granulosa cell tumors (GCTs) weakly expressed this protein; in contrast, PLZF protein expression was not significantly different between both study groups. PMID:25023763
Statistically significant differences in staining patterns were found for histone H1.5, distinguishing leiomyosarcomas from leiomyomas. PMID:24784718
Promyelocytic leukemia zinc finger and histone H1.5 differentially stain low- and high-grade pulmonary neuroendocrine tumors PMID:23416030
FoxP3 interacts with H1.5 to alter its binding to target genes to modulate their expression and to program Treg function. PMID:21654845
the mode of the chromatin fiber compaction changes depending both on salt environment and linker histone H1 PMID:16185066
Phosphorylation of human H1 variants occurs nonrandomly during both interphase and mitosis and distinct serine- or threonine-specific kinases are involved in different cell cycle phases. PMID:16377619
Phosphorylation at threonine 10 appears in prometaphase and disappears in telophase, and that this hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin condensation is maximal. PMID:19136008

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Subcellular Location

Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.

Protein Families

Histone H1/H5 family

Tissue Specificity

Ubiquitous. Expressed in the majority of the cell lines tested and in testis.

Database Links

HGNC: 4719

UNIGENE: Hs.131956

KEGG: hsa:3009

STRING: 9606.ENSP00000330074

OMIM: 142711

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