GPRC6A Antibody

Code: CSB-PA722571LA01HU

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20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q5T6X5

Target Names

GPRC6A

Alternative Names

bA86F4.3 antibody; G protein coupled receptor 33 antibody; G protein coupled receptor family C group 6 member A antibody; G-protein coupled receptor family C group 6 member A antibody; G-protein coupled receptor GPCR33 antibody; GPC6A_HUMAN antibody; GPCR 33 antibody; GPCR 6A antibody; GPCR antibody; GPCR33 antibody; GPCR6A antibody; GPR C6A antibody; GPRC 6A antibody; gprc6a antibody; hGPCR 33 antibody; hGPCR33 antibody; hGPRC6A antibody; OTTHUMP00000017075 antibody; Predicted with SOSUI analysis antibody; seven transmembrane helix receptor antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human G-protein coupled receptor family C group 6 member A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate
All lanes: GPRC6A antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 105, 85, 97 kDa
Observed band size: 105 kDa
IHC image of CSB-PA722571LA01HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC-3 cells with CSB-PA722571LA01HU at 1:100, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cells with CSB-PA722571LA01HU at 1:100, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Target Background

Function(From Uniprot)

Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalcin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids.

Gene References into Functions

If the regulatory functions of GPRC6A identified in mice translate to humans, and polymorphisms in GPRC6A are found to predict racial disparities in human diseases, GPRC6A may be a new gene target to predict, prevent, and treat MetS, PCa, and other disorders impacted by GPRC6A. PMID:28180060
Human GPRC6A is a functional osteocalcin and testosterone sensing receptor that promotes prostate cancer progression. PMID:28659174
he nonrare polymorphism rs2274911 in the GPRC6A gene was associated with insulin resistance features, independently of the metabolic phenotype and osteocalcin levels. PMID:27696500
Genetic Variations in the Human G Protein-coupled Receptor Class C, Group 6, Member A (GPRC6A) Control Cell Surface Expression and Function PMID:27986810
Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A. PMID:27673554
results suggest that G protein-coupled receptor class C group 6 member A(GPRC6A) inactivation or sub-function contributes to reduced exposure to androgens, leading to cryptorchidism during fetal life and/or low sperm production in adulthood PMID:26735260
association of the THADA, FOXP4, GPRC6A/RFX6 and 8q24 genes with prostate cancer in Asian populations. PMID:26537068
The present experiments demonstrated a critical role for the GPRC6A-ERK1/2 and PI3K/Akt signaling pathway in arginine-mediated fibroblast survival. PMID:24651445
Duox1 activity is stimulated by testosterone through GPRC6A in skin keratinocytes PMID:25164816
The F464Y substitution in GPRC6A may be a cause of primary testicular failure. PMID:23728177
Extracellular Ca2+ activates the NLRP3 inflammasome via CaSR and GPRC6A. PMID:23271661
the GPRC6A receptor functions as an amino acid sensor in GLUTag cells that promotes GLP-1 secretion PMID:23269670
Our results provide further support for association of the C2orf43, FOXP4, GPRC6A and RFX6 genes with prostate cancer in Eastern Asian populations PMID:22662242
GPRC6A is a novel molecular target for regulating prostate growth and cancer progression. PMID:21681779
Observational study of gene-disease association. (HuGE Navigator) PMID:20654748
Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID:20676098
that GPRC6A gene polymorphisms were significantly associated with human spine BMD. PMID:19874200
GPRC6A is widely expressed in brain and peripheral tissues with highest levels in kidney, skeletal muscle, testis, and leucocytes. PMID:15194188
elucidating agonist interaction with GPRC6A, a novel family C G-protein-coupled receptor. PMID:15576628
GPRC6A is a cation-, calcimimetic-, and osteocalcin-sensing receptor PMID:16199532

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 3 family

Tissue Specificity

Isoform 1 is expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not detec

Database Links

HGNC: 18510

UNIGENE: Hs.266745

KEGG: hsa:222545

STRING: 9606.ENSP00000309493

OMIM: 613572

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