GPR176 Antibody

CSB-PA618889LA01HU

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20μg50μg100μg

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q14439

Target Names

GPR176

Alternative Names

GPR176; G-protein coupled receptor 176; HB-954

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human G-protein coupled receptor 176 protein (321-515AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: GPR176 antibody at 10µg/ml + A431 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57, 53 kDa
Observed band size: 57 kDa
Immunofluorescent analysis of A431 cells using CSB-PA618889LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Description

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Target Background

Function

Orphan receptor involved in normal circadian rhythm behavior. Acts through the G-protein subclass G(z)-alpha and has an agonist-independent basal activity to repress cAMP production.

Gene References into Functions

Data suggest that Gpr176 is an SCN-(suprachiasmatic nucleus-)enriched orphan GPCR (G-protein-coupled receptor) that sets the pace of circadian behavior. [REVIEW] PMID:28502923

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Database Links

HGNC: 32370

UNIGENE: Hs.37196

KEGG: hsa:11245

STRING: 9606.ENSP00000453076

OMIM: 612183

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