GPC4 Antibody

Code: CSB-PA009706LA01HU

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20μg50μg100μg

US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Target Background

Product Details

Uniprot NO.

O75487

Target Names

GPC4

Alternative Names

dJ900E8.1 glypican 4 antibody; Glypican proteoglycan 4 antibody; Gpc4 antibody; GPC4_HUMAN antibody; K Glypican antibody; K-glypican antibody; Kglypican antibody; OTTHUMP00000024052 antibody; Secreted glypican 4 antibody; Secreted glypican-4 antibody; UNQ474/PRO937 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Glypican-4 protein (19-210AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: GPC4 antibody at 8µg/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63, 55 kDa
Observed band size: 63 kDa
Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA009706LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA009706LA01HU at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA009706LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Description

In the production of the GPC4 polyclonal antibody, a rabbit was initially immunized with the recombinant human Glypican-4 protein (19-210aa). Afterward, the antibodies were obtained and extracted from the rabbit serum, and subsequently purified using protein G affinity chromatography.

The resulting GPC4 polyclonal antibody possesses a purity level exceeding 95%, indicating that it contains almost no impurities. It has been demonstrated to recognize and bind to GPC4 protein in human and mouse samples. In addition, the versatility of the GPC4 polyclonal antibody has been demonstrated through its successful validation in various applications, such as ELISA, WB, IHC, and IF, indicating its effectiveness in a range of experimental settings.

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Citations

GSDME unlocks astrocyte-driven neurotoxicity in Alzheimer's Disease X Xie, C Ji, J Xu, X Lu, G Guo, W Liu, X Wu, Y Chen,/,2025

Target Background

Function(From Uniprot)

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.

Gene References into Functions

The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma. PMID:27071854
According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures. PMID:27425250
serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk PMID:26486309
Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness). PMID:23633195
new function of miR-125a by targeting gene glypican-4 in cell growth process PMID:22644326
Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate. PMID:22761013