GDF10 Antibody

Code: CSB-PA009343LA01HU

Rate （5.0）
Review （1）
Citation （1）

Size:

20μg50μg100μg

US$83

Quantity:

Species Reactivity: Human, Rat

Raised in: Rabbit

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

P55107

Target Names

GDF10

Alternative Names

BIP antibody; BMP 3b antibody; BMP-3b antibody; BMP3B antibody; BMP3B_HUMAN antibody; Bone inducing protein antibody; Bone morphogenetic protein 3b [Precursor] antibody; Bone morphogenetic protein 3b antibody; Bone-inducing protein antibody; GDF 10 antibody; GDF-10 antibody; Gdf10 antibody; growth differentiation factor 10 antibody; Growth/differentiation factor 10 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Growth/differentiation factor 10 protein (369-478AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Rat brain tissue
All lanes: GDF10 antibody at 4.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
IHC image of CSB-PA009343LA01HU diluted at 1:200 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA009343LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Description

The rabbit anti-human GDF10 polyclonal antibody is an IgG antibody produced in rabbits that specifically recognizes and binds to the human and rat GDF10 protein. Its immunogen is the recombinant human GDF10 protein (369-478aa). Protein G purification of this GDF10 antibody brought it a purity of up to 95%. The specificity and reliability of this GDF10 antibody have been verified in ELISA, WB, and IHC applications.

GDF10 plays a crucial role in the regulation of cell growth, differentiation, and development in various tissues and organs. It especially promotes cell differentiation in the skeletal and nervous systems. It is also involved in the development and maintenance of bone and cartilage, as well as in the formation and repair of neural tissue.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample dilution: 1: 1000

Review: Western blots and quantification of ATRX, GDF10 and Lingo1 expression levels in the DCN of the tinnitus and non‑tinnitus groups and the control group are presented. GDF10 levels increased significantly at 3 weeks after noise exposure in the tinnitus group. data are presented as the mean ± SEM, as determined by one-way ANOVA followed by Tukey's post-hoc test.

By Anonymous

Citations

Axonal sprouting in the dorsal cochlear nucleus affects gap‑prepulse inhibition following noise exposure Kyu‑Hee Han, et al,/,2019

Target Background

Function(From Uniprot)

Growth factor involved in osteogenesis and adipogenesis. Plays an inhibitory role in the process of osteoblast differentiation via SMAD2/3 pathway. Plays an inhibitory role in the process of adipogenesis.

Gene References into Functions

Study shows that GDF10 is down-regulated in patients with oral squamous cell carcinoma, and is an independent risk factor for overall survival. Its expression is regulated by TGFBR3 which shares the signaling inhibiting epithelial-mesenchymal transition. PMID:25728212
GDF10 is a stroke-induced signal for axonal sprouting and functional recovery. PMID:26502261
new hypoxia-inducible and SOX9-regulated genes, Gdf10 and Chm-I. In addition, Mig6 and InhbA were induced by hypoxia, predominantly via HIF-2alpha PMID:18077449
BMP3b and BMP6 genes were suppressed by DNA methylation and methylation of BMP3b is significantly frequent in Japanese malignant pleural mesotheliomas (MPMs), suggesting its pathogenic role and the ethnic difference in MPMs. PMID:18949431