Efnb3 Antibody

Code: CSB-PA007467LA01MO

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US$83

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Species Reactivity: Mouse, Human

Raised in: Rabbit

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Uniprot NO.

O35393

Target Names

Efnb3

Alternative Names

Efnb3Ephrin-B3 antibody

Raised in

Rabbit

Species Reactivity

Mouse, Human

Immunogen

Recombinant Mouse Ephrin-B3 protein (28-227AA)

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: Efnb3 antibody at 12µg/ml + HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa

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Target Background

Function(From Uniprot)

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.

Gene References into Functions

EphA2 mAb treatment could partially inhibit LPSinduced inactivation of EphBEphrin B3 signalling, while Ephrin B3 overexpression could abrogate LPSinduced activation of EphA2Ephrin A1 signalling. EphB1/Ephrin B3 signalling may antagonise the EphA2/Ephrin A1dependent pathway following LPS treatment PMID:29901151
Data suggest that molecules in the erythropoietin-producing hepatocellular receptor B family (EPHB) / ephrinB (EFNB) signalling pathways, specifically ephrin B3 and GRIP1, are involved blood pressure regulation. PMID:27941904
ephrin B3/EphB2 are obvious candidates for driving the Syk-dependent repulsive response. PMID:27122172
The Eph/ephrin-B3-mediated heterotypic or homotypic cell interactions between thymocytes and thymic epithelial cells (TECs), or between TECs and themselves, contribute to the early maturation of MTS20(+) TECs. PMID:27060907
Findings suggest that EphrinB3 may be a relevant target for promoting remyelination in demyelinating disease PMID:26687980
The morphological and behavioural abnormalities in ephrin-B3 mutant mice are rescued by conditional knock-in of wild-type ephrin-B3 during the critical period when axon targeting and fear responses are initiated. PMID:27008987
Ephrin-B3 knockout mice had significantly reduced pre-pulse inhibition compared with controls. PMID:24949848
Conclude that EphB3 mediates cell death in the adult cortex through a novel dependence receptor-mediated cell death mechanism in the injured adult cortex and is attenuated following ephrinB3 stimulation. PMID:24810043
findings indicate that a single guidance system, ephrinB3/EphA4, controls the formation of ascending and descending longitudinal axons in the spinal cord. PMID:24360544
these data introduce EphB3 as a new biomarker to identify beta-cells at a critical step during their step-wise differentiation and define the timeframe of endocrine differentiation. PMID:22434763
Induction of experimental stroke results in enhanced cell proliferation and neuronal differentiation around the lesion site of ephrin-B3-deficient compared to ephrin-B3-expressing mice. PMID:21779764
EphrinB3 contributes to myelin-derived axonal growth inhibition and limits recovery from adult central nervous system trauma. PMID:22411787
cell contact-mediated bidirectional ephrinB3/EphA4 signaling mediates the sorting of medial ganglionic eminence- and preoptic area-derived interneurons in the deep and superficial migratory stream. PMID:22171039
This study demonistrated tht ephrin-B reverse signaling into three distinct intracellular pathways and protein-protein interactions that mediate the maturation of postsynaptic neurons. PMID:21964490
These studies support an anti-proliferative role for EphB3 in regulating neural stem/progenitor cell numbers in the developing subventricular zone. PMID:20969945
found that ablation of ephrin-B3 results in an enhancement of the NMDA receptor component of synaptic transmission relative to the AMPA receptor component in CA1 synapses PMID:20678574
Ephrinb3 is directly involved in the specificity of mesostriatal projection. PMID:20719236
conclude that ephrinB3-induced EphA4 signaling repels some components of the mammalian spinal central pattern generators (CPG) and restricts their axonal projections to one side of the spinal cord PMID:12649481
Organ of Corti and spiral ganglion showed strong expression of ephrin-A3, ephrin-B2 and ephrin-B3. PMID:12684176
EFNB3-Fc in the presence of suboptimal anti-CD3 crosslinking enhanced T-cell responses in terms of proliferation, activation marker expression, interferon-gamma but not interleukin-2 production, and cytotoxic T-cell activity PMID:13679370
Ephrin-B3 is a myelin-based inhibitor of neurite outgrowth. PMID:16020529
May act like a receptor by transducing reverse signals in order to regulate the number of synapses formed in the hippocampus. May stimulate forward signaling to modulate a number of other proteins involved in synaptic activity, learning, and memory. PMID:16466709
Data show that ephrinB3 contributes to the control of cell proliferation and survival in the adult subventricular zone. PMID:16483793
Novel mechanism for independent modulation of shaft synapses through ephrinB3 reverse signaling. PMID:17626212
Rac-GAP alpha-chimerin regulates motor-circuit formation as a key mediator of Efnb3 forward signaling. PMID:17719550
A previously unknown pathway is revealed that controls axon pruning by which ephrin-B3 reverse signals regulate actin dynamics to bring about the retraction of growth cones. PMID:19182796

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Ephrin family

Tissue Specificity

Expressed on lateral floor plate cells, specifically on commissural axon segments that have passed through the floor plate. Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed in myogenic progeni

Database Links

UNIGENE: Mm.249637

KEGG: mmu:13643

STRING: 10090.ENSMUSP00000004036

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