CLEC12A Antibody

Code: CSB-PA725176ESR1HU

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20μl50μl100μl

US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q5QGZ9

Target Names

CLEC12A

Alternative Names

C-type lectin domain family 12 member A antibody; C-type lectin-like molecule 1 antibody; CL12A_HUMAN antibody; CLEC12A antibody; CLL-1 antibody; DCAL-2 antibody; Dendritic cell-associated lectin 2 antibody; MICL antibody; Myeloid inhibitory C-type lectin-like receptor antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human C-type lectin domain family 12 member A protein (66-265AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: C-type lectin domain family 12 member A antibody at 2μg/ml + Mouse skeletal muscle tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 31, 33, 9, 28, 25 kDa
Observed band size: 31 kDa
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA725176ESR1HU at dilution of 1:100

Description

The CLEC12A antibody was raised in rabbits against a partial recombinant protein corresponding to the 66-265 amino acids of the human CLEC12A protein. It exists as an unconjugated IgG isoform. It underwent antigen affinity purification.

This CLEC12A polyclonal antibody has undergone rigorous quality control in multiple applications, including ELISA, WB, and IHC. And it shows reactivity with human and mouse CLEC12A protein. The CLEC12A protein mainly modulates the immune response, particularly in the regulation of myeloid cell activation and differentiation. It is also involved in the pathogenesis of certain cancers and autoimmune diseases.

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Target Background

Function(From Uniprot)

Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases.

Gene References into Functions

Enumeration of circulating hMICL+ stem cells by flow cytometry can discriminate between myeloproliferative neoplasm phenotypes and holds potential for monitoring disease evolution. PMID:29427319
CLL-1 is a novel prognostic predictor that could be exploited to supplement the current acute myeloid leukemia prognostic risk stratification system. PMID:28810819
The CLEC12A is an effective new candidate with great potential for in vivo Ag delivery into mDCs and pDCs, thereby using the specialized functions and cross-talk capacity of these DC subsets to boost tumor-reactive T cell immunity in cancer patients. PMID:27566820
Due to the absence of CLEC12A on normal haematopoietic stem cells, CLEC12A stem cell immunophenotyping may contribute to diagnosing and monitoring MDS patients and could furthermore add knowledge about disease propagating cells in MDS. PMID:27612176
We propose the hypothesis that decreased expression of CLEC12A is a common denominator in the hyperinflammatory responses observed in Behcet's syndrome and gout. PMID:25957656
Upon CLL1-selective binding of this fusion protein, granulocytes acquire additional TRAIL-mediated cytotoxic activity that, importantly, potentiates antibody-mediated cytotoxicity of clinically used therapeutic antibodies PMID:25760768
We conclude the hMICL/CD123-based MFC assay is a promising MRD tool in AML. PMID:24152218
There is a potential genetic association of CLEC12A with rheumatoid arthritis (RA). Since CLEC12A encodes for the myeloid inhibitory C-type lectin-like receptor that modulates cytokine synthesis, this receptor may contribute to the pathogenesis of RA. PMID:22341585
For production of cytotoxic T cells, transgenic DEC-205 and Clec9A, but not Clec12A, are effective targets for antibody-mediated delivery of antigens for vaccination, although only in the presence of adjuvants. PMID:21677141
MICL is a negative regulator of granulocyte and monocyte function PMID:14739280
the molecular cloning, tissue distribution, and functional characterization of a novel NK cell receptor, KLRL1, from human and mouse dendritic cells [KLRL1] PMID:15238421
Expression cloning identified the antigen recognized as C-type lectin-like molecule-1, a previously undescribed transmembrane glycoprotein PMID:15548716
Dendritic-cell (DC)-associated C-type lectin receptors (CLRs) take up antigens to present to T cells and regulate DC functions. PMID:16239426
human MICL may be involved in the control of myeloid cell activation during inflammation PMID:16838277
CLL-1 expression is also present on the CD34(+)CD38(-) stem- cell compartment in acute myeloid leukemia PMID:17609428
mMICL recognises an endogenous ligand in a variety of murine tissues, suggesting that the receptor plays a role in homeostasis PMID:18350551
Anti-Clec12A mAb alone produced only moderate responses, but these were amplified by coinjecting only small amounts of LPS as a dendritic cell activation agent PMID:19494282

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Subcellular Location

Cell membrane; Single-pass type II membrane protein. Note=Ligand binding leads to internalization.

Tissue Specificity

Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leuko

Database Links

HGNC: 31713

UNIGENE: Hs.190519

KEGG: hsa:160364

STRING: 9606.ENSP00000347916

OMIM: 612088

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