| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IHC | 1:20-1:200 |
Western blot
All lanes: CHID1 antibody at 0.2μg/ml
Lane 1: Hela whole cell lysate
Lane 2: U251 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 45, 48, 42 kDa
Observed band size: 45 kDa
Immunohistochemistry analysis of human breast cancer using CSB-PA883614LA01HU at dilution of 1:100
Immunohistochemistry analysis of human pancreatic tissue using CSB-PA883614LA01HU at dilution of 1:100
Western Blot
Positive WB detected in: U251 whole cell lysate, 293 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate
All lanes: CHID1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45, 48, 42 kDa
Observed band size: 45 kDa
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