CEP250 Antibody

Code: CSB-PA871581LA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, IF

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Target Background

Product Details

Uniprot NO.

Q9BV73

Target Names

CEP250

Alternative Names

CEP250 antibody; CEP2 antibody; CNAP1Centrosome-associated protein CEP250 antibody; 250 kDa centrosomal protein antibody; Cep250 antibody; Centrosomal Nek2-associated protein 1 antibody; C-Nap1 antibody; Centrosomal protein 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Centrosome-associated protein CEP250 protein (40-293AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescent analysis of Hela cells using CSB-PA871581LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Target Background

Function(From Uniprot)

May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase.

Gene References into Functions

C-NAP1-null cells were viable and had an increased frequency of premature centriole separation, accompanied by reduced density of the centriolar satellites, with reexpression of C-NAP1 rescuing both phenotypes. Centrosome amplification induced by DNA damage or by PLK4 or CDK2 overexpression was markedly reduced in the absence of C-NAP1. PMID:28100636
ASPP1/2 interacted with centrosome linker protein C-Nap1. Co-depletion of ASPP1 and ASPP2 inhibited re-association of C-Nap1 with centrosome at the end of mitosis. PMID:25660448
multisite phosphorylation precipitates centrosome disjunction at the onset of mitosis PMID:24695856
A homozygous nonsense CEP250 mutation, in combination with a heterozygous C2orf71 nonsense mutation, causes an atypical form of Usher syndrome, characterised by early-onset sensorineural hearing loss and a relatively mild retinitis pigmentosa. PMID:24780881
Centlein complexes with C-Nap1 and Cep68 at the proximal ends of centrioles during interphase. PMID:24554434
C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis. PMID:23070519
Data show that the dissociation of C-Nap1 from mitotic centrosomes is regulated by localized phosphorylation rather than generalized proteolysis. PMID:12140259
CEP135 acts as a platform protein for C-NAP1 at the centriole. PMID:18851962

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Subcellular Location

Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, cilium basal body. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment. Note=Component of the core centrosome. In interphase cells, it specifically associates with the proximal ends of both mother and daughter centrioles. Associates with the centrosome in interphase cells. In mitotic cells, it dissociates from the mitotic spindle poles. At the end of cell division, it reaccumulates at centrosomes.

Tissue Specificity

Ubiquitously and weakly expressed.

Database Links

HGNC: 1859

UNIGENE: Hs.443976

KEGG: hsa:11190

STRING: 9606.ENSP00000380661

OMIM: 609689

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