CASP7 Antibody

Code: CSB-PA15479A0Rb

Rate （5.0）
Review （1）
Citation （1）

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF, IP

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Product Details
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Target Background

Product Details

Uniprot NO.

P55210

Target Names

CASP7

Alternative Names

apoptosis-related cysteine peptidase antibody; Apoptotic protease Mch-3 antibody; CASP-7 antibody; CASP7 antibody; CASP7_HUMAN antibody; Caspase-7 subunit p11 antibody; CMH-1 antibody; ICE-LAP3 antibody; ICE-like apoptotic protease 3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Caspase-7 protein (24-198AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Immunoprecipitation (IP) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: CASP7 antibody at 2µg/ml
Lane 1: A549 whole cell lysate
Lane 2: HGC-27 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 35, 29, 38, 32 kDa
Observed band size: 35 kDa
IHC image of CSB-PA15479A0Rb diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA15479A0Rb diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-PA15479A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CASP7 in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA15479A0Rb in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA15479A0Rb (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Western blot

Sample type: cells

Review: Effects of PRE, PRO, and SYN on cognitive impairment of DSS-induced colitis mice via regulation of apoptosis pathway. Western blot band images:and the expression levels of Bcl2, Bax, Bax/Bcl2 ratio, and caspase 7.

By Anonymous

Citations

Anti-Amnesic Effect of Synbiotic Supplementation Containing Corni fructus and Limosilactobacillus reuteri in DSS-Induced Colitis Mice HL Lee,/,2022

Target Background

Function(From Uniprot)

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death. Cleaves phospholipid scramblase proteins XKR4, XKR8 and XKR9.; Lacks enzymatic activity.

Gene References into Functions

Pyrin signaling is dispensable for Clostridium difficile infection (CDI) associated intestinal epithelial cells death and for in vivo pathogenesis; C. difficile enterotoxins induce activation of executioner caspases 3/7 via the intrinsic apoptosis pathway indicating that caspase-3/7-mediated intestinal epithelial cells apoptosis is critical for in vivo host defense during early stages of CDI. PMID:30451870
CASP8: rs1045494 (C > T), PIK3R1: rs3756668 (A > G) and CASP7: rs4353229 (T > C), were associated with longer overall survival in limited disease-small cell lung cancer patients after chemoradiotherapy PMID:26988918
Results show that caspase-7 is phosphorylated by PAK2 at S239 which inhibits its activity by two divergent mechanisms prior to and following caspase activation: initial phosphorylation allosterically slows activation by upstream initiator caspases by impeding cleavage at the intersubunit linker and a second phosphorylation site directly blocks substrate binding. PMID:27889207
Caspase-7 regions involved in the interaction with Hsp90 co-chaperone p23 and substrate recognition. PMID:28863261
Study provides evidence that a loss-of-function variant rs10553596 in CASP7 associates with significantly reduced Alzheimer's disease incidence in elder populations. PMID:27358062
Low expression of CASP7 is associated with non-small cell lung cancer. PMID:26307684
Compared with the TT genotype, the rs10787498GT genotype was associated with an increased cervical cancer risk. PMID:25784056
Phosphorylate caspase-7 bu Src at multiple tyrosine sites enhanced its cellular apoptotic effect. PMID:24407236
Potentially functional polymorphisms in the CASP7 gene contribute to gastric adenocarcinoma susceptibility in an eastern Chinese population. PMID:24040159
showed that CASP7 is downregulated in primary prostate tumors and metastatic lesions across multiple data sets PMID:22986525
Genetic variations of CASP7 may modulate overall survival and progress-free survival of patients with advanced non-small cell lung cancer treated with platinum-based chemotherapy. PMID:22441531
genetic polynorphism is associated with the risk of childhood leukemia PMID:22548721
XIAP does not function as a NEDD8-E3 ligase for caspase-7 in vivo PMID:22584050
Cellular expression of caspase-7 lacking the critical lysine residues resulted in less-efficient PARP and p23 cleavage compared with cells expressing the wild-type peptidase. PMID:22451931
identify serine 118 in the transactivation domain of YY1 as the site of CK2alpha phosphorylation, proximal to a caspase 7 cleavage site PMID:22184066
Caspase-7 cleaves human TERT at residues E286 and D628 as part of the apoptosis pathway in cultured cells. PMID:21936563
highly expressed PAK2 mediates chemotherapeutic resistance in human breast invasive ductal carcinoma by negatively regulating caspase-7 activity PMID:21555521
The RIPK1 and CASP7 polymorphisms can be considered as possible prognostic markers for survival after curative resection in patients with colorectal cancer. PMID:20567846
Caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes. PMID:21157428
Single Nucleotide Polymorphisms in CASP7 is asspciated with lung cancer. PMID:20661084
caspase 7 cleavage of ORF57 may represent a cellular function against lytic KSHV infection PMID:20159985
Specifically interfering with caspase-7 activation may hold therapeutic value for the treatment of cancer and inflammatory ailments. PMID:19782763
Pro-CASP7 was detected in mitochondria, cytosol, nucleus, and microsomes of U937 cells. During TPA-induced differentiation, it moved to the mitochondria. PMID:12145703
caspase 9 by itself can activate caspase 7 in the absence of the caspase 3-dependent pathway in TNF-alpha-induced apoptosis PMID:12804035
the N-peptide of caspase 7 serves to physically sequester the caspase-7 zymogen in a cytosolic location that prevents access by upstream activators PMID:12824163
our data suggest that the inactivating mutations of the CASPASE-7 gene might lead to the loss of its apoptotic function and contribute to the pathogenesis of some human solid cancers PMID:12970753
caspase-7 is involved earlier than other effector caspases in apoptosis PMID:14583630
cleavage of Claspin by caspase-7 inactivates the Chk1 signaling pathway PMID:16123041
Caspase 7 activation is a prominent feature in periodontitis-associated tissue injury. PMID:16213496
Promoters of CASP7 genes are modulated by prohibitin. PMID:16918502
AIF overexpression specifically resulted in the activation of caspase-7, thereby amplifying the inhibition of protein synthesis including eIF3g cleavage. PMID:17094969
observation indicates that neither CASP7 nor CASP8 mutation may occur in gastrointestinal lymphomas, and suggests that neither of them may play an important role in the development of gastrointestinal lymphomas PMID:17532763
crystal structures show that the S2 pocket of caspase-7 can accommodate diverse residues PMID:17697120
A reticulon protein is identified as one out of a selected number of caspase targets during apoptosis and as a novel substrate for Cdk1 and 2.[ PMID:18072206
These results revealed that caspase-7 has a novel role during cell cycle progression at mitosis. PMID:18459962
Valosin-containing protein was cleaved by both capspase-7 and caspase-3 in vitro and during apoptosis; degradomic approach to caspase-7 provides new candidate substrates and valuable clues to the specific function of caspase-7 in apoptosis PMID:18596415
CASP7 (caspase 7) rs2227309 SNP was not associated with rheuatoid arthritis (RA) in a European Caucasian population. CASP7 isoforms alpha and beta could have an involvement in the apoptosis process in RA PMID:18785314
Modulation of effector caspase-7 cleavage determines response of breast and lung tumor cell lines to chemotherapy. PMID:19241192
Results show that caspase 7, as an SREBP-1/2 target, can be induced under mevalonate-restricting conditions, which might help overcome its shortage. PMID:19323650
These findings suggest that genetic variants in caspase-3 and caspase-7 may play a role in endometrial cancer susceptibility. PMID:19531679
Dissecting an allosteric switch in caspase-7 using chemical and mutational probes. PMID:19581639
there is an an association between HCV core and HAX-1, which promotes 5-FU mediated p53-dependent caspase-7 activation and hepatocyte growth inhibition PMID:19605487