CASP12 Antibody

Code: CSB-PA747641LA01HU

Rate （5.0）
Review （2）
Citation （3）

Size:

20μg50μg100μg

US$83

Quantity:

Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q6UXS9

Target Names

CASP12

Alternative Names

CASP 12 antibody; CASP-12 antibody; Casp12 antibody; CASP12P1 antibody; caspase 12 (gene/pseudogene) antibody; caspase 12 pseudogene 1 antibody; CASPC_HUMAN antibody; Inactive caspase-12 antibody; OTTHUMP00000207032 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Inactive caspase-12 protein (61-282AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: CASP12 antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 20, 30 kDa
Observed band size: 39 kDa
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA747641LA01HU at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA747641LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 2 reviews

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Applications : Western blot assays

Sample type: Human cells

Review: Cleavage of caspase 12 was detected by Western blot. Combined treatment with tBHP and HDACi induced apoptosis in HCC cells. Apoptosis of HCC cells after treatment with tBHP (25 μmol/L), SAHA (1 mol/L), MS275 (1 μmol/L), or combinatorial treatment with tBHP and HDACi.

By Anonymous

Applications : Western blots

Sample type: cells

By Anonymous

Citations

Target Background

Function(From Uniprot)

Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.

Gene References into Functions

Piezo1 plays an important role in the apoptosis of human osteoarthritis-derived chondrocytes through a caspase-12-dependent pathway. PMID:28731145
study indicated the potential effect of Casp12 was on the activation of NF-kappaB by the degradation of IkappaBalpha in NPC cells. PMID:28380444
CASP12 genotype thus does not influence the phenotype of systemic lupus erythematosus in African-Americans PMID:26973091
Endoplasmic reticulum stress may be associated with apoptosis of LECs, resulting in cataract formation in diabetic patients. PMID:27130368
Endoplasmic reticulum stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy. PMID:26261584
Expression of caspase-12 was linked to decreased systemic and adipose tissue inflammation in a cohort of African American obese children. PMID:26582949
We found that RNA interference-induced Caspase-12 silencing increased NOD1, hBD1 and hBD2 expression PMID:25503380
conclude that the functional CASP12L allele is not a marker for susceptibility and/or severity of CAP PMID:24586588
EtOH-induced activation of caspase-12 could be one of the underlying mechanisms of hepatocyte apoptosis. PMID:24224909
Distribution of CASP12 alleles (#rs497116) in African-Americans (AA) with rheumatoid arthritis (RA). There was no difference in the overall distribution of genotypes within AA with RA, but homozygous patients had lower baseline joint-narrowing scores. PMID:24515649
CASPASE-12 does not influence the susceptibility to Candida sepsis, nor has any effect on the serum cytokine concentrations in Candida sepsis patients during the course of infection. PMID:21706251
examines the role that CASP12 plays in down-regulating inflammation, we hypothesize that pathogens which exploit the inflammatory response are restrained by an active CASP12 gene product PMID:21872999
caspase 12 has acquired deleterious mutations PMID:12054529
Pathways involving this enzyme are involved in a neurodegenerative disease in vivo, and thus offer novel potential targets for the treatment of prion disorders. PMID:14532116
Na/Ca exchanger overexpression activates caspase-12 PMID:15033764
Csp12-L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis PMID:15129283
Observational study of genotype prevalence. (HuGE Navigator) PMID:16917906
The activation of caspase-9 and depolarization of mitochondrial membrane potential were induced by BIK, which were decreased concomitant with caspase-12 silenced. PMID:17574210
proteolytic activity of caspase-12 is confined to its own proenzyme PMID:18332441
hRPE cells express a high level of caspase-12S. The regulated expression of caspase-12S suggests that this caspase recruitment domain (CARD)-only protein may be an endogenous dominant negative regulator that modulates inflammatory responses in hRPE cells. PMID:18791174
estrogen-mediated inhibition of Csp-12L expression is a built-in mechanism that has evolved to protect females from Listeria monocytogenes infection. PMID:19447924
the NOD2/RIP2 pathway has a role in recognition of Yersinia, but caspase-12 does not modulate innate host defense against Y. pestis PMID:19721713
The calcium-calpain-caspase-12-caspase-3 cascade is altered in F508del-CFTR expressing cells. PMID:20041182
Reactive oxygen species promote caspase-12 expression and tubular apoptosis in diabetic nephropathy. PMID:20299359
Observational study of gene-disease association. (HuGE Navigator) PMID:20299368
Observational study of gene-disease association. (HuGE Navigator) PMID:20423816
Observational study of gene-disease association. (HuGE Navigator) PMID:20577092
Constitutively active forms of caspase-12 (rev-Deltapro1 and rev-Deltapro2) could induce cell death in cells transfected with the corresponding expression vectors. PMID:20646990