BCKDK Antibody

Code: CSB-PA002607ESR2HU

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20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

O14874

Target Names

BCKDK

Alternative Names

[3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody; BCKD kinase antibody; BCKD-kinase antibody; BCKD_HUMAN antibody; BCKDHKIN antibody; Bckdk antibody; BCKDKD antibody; BDK antibody; Branched chain alpha keto acid dehydrogenase kinase antibody; Branched chain ketoacid dehydrogenase kinase antibody; Branched-chain alpha-ketoacid dehydrogenase kinase antibody; mitochondrial antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial protein (153-412AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:200-1:1000
IHC	1:20-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: BCKDK antibody at 5µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47, 38, 42 kDa
Observed band size: 47 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA002607ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA002607ESR2HU at dilution of 1:100

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function(From Uniprot)

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Gene References into Functions

our study indicates that BCKDK promotes colorectal tumorigenesis through up-regulation of the MAPK signaling pathway by phosphorylating MEK at Ser221. PMID:28501528
There is a relationship between plamsa CST and BCKDK in sepsis patients. PMID:27773658
Results demonstrate that BCKDK mutations can result in neurobehavioral deficits in humans. PMID:24449431
This publication is about the mouse gene but contains the 5' sequence of the human gene, including upstream open reading frame information. PMID:15302860
employment of multidimensional heteronuclear NMR techniques to determine the structure and dynamics of the LBD of the human branched-chain alpha-keto acid dehydrogenase complex (hbLBD) PMID:11839747