Acetyl-HIST1H2BB (K16) Antibody

CSB-PA010402OA16acHU

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Size:

20μl50μl100μl

US$88

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, ICC, IF, ChIP

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Target Background

Product Details

Uniprot NO.

P33778

Target Names

HIST1H2BB

Alternative Names

HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence around site of Acetyl-Lys (16) derived from Human Histone H2B type 1-B

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, ICC, IF, ChIP

Recommended Dilution

Application	Recommended Dilution
ICC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Immunofluorescence (IF) Protocol Native Chromatin Immunoprecipitation(ChIP) Protocol

Datasheet & COA

Antibody FAQs

Images

Immunocytochemistry analysis of Hela cells using CSB-PA010402OA16acHU at dilution of 1:100
Immunofluorescent analysis of Hela cells treated with NaB using CSB-PA010402OA16acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2BB (CSB-PA010402OA16acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Description

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Target Background

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene References into Functions

Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID:29643390
RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID:26854224
RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID:21362548

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Histone H2B family

Database Links

HGNC: 4751

UNIGENE: Hs.553494

KEGG: hsa:3018

STRING: 9606.ENSP00000350580

OMIM: 602803

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