ATG4A Antibody

Code: CSB-PA845174EA01HU

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20μg50μg100μg

US$83

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Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Uniprot NO.

Q8WYN0

Target Names

ATG4A

Alternative Names

AI627006 antibody; APG4 autophagy 4 homolog A antibody; Apg4a antibody; ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody; ATG4 autophagy related 4 homolog A antibody; ATG4A antibody; ATG4A_HUMAN antibody; Atg4al antibody; AUT like 2 cysteine endopeptidase antibody; AUT-like 2 cysteine endopeptidase antibody; Autl2 antibody; Autophagin 2 antibody; Autophagin-2 antibody; Autophagy related 4A cysteine peptidase antibody; Autophagy related cysteine endopeptidase 2 antibody; Autophagy related protein 4 homolog A antibody; Autophagy-related cysteine endopeptidase 2 antibody; Autophagy-related protein 4 homolog A antibody; AV169859 antibody; Cysteine protease ATG4A antibody; hAPG4A antibody; MGC107179 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Cysteine protease ATG4A protein (1-214AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:200-1:1000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: ATG4A antibody at 2µg/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 46, 39, 37, 43 kDa
Observed band size: 46 kDa

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Target Background

Function(From Uniprot)

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene References into Functions

An intron SNP rs807185 in ATG4A might be a protective factor for lung cancer. PMID:26061994
ATG4A promotes the metastasis of gastric cancer cells via the Notch signaling pathway, which is an autophagy-independent mechanism PMID:27276686
The structure and regulatory machinery of Atg4a [review] PMID:28253956
We conclude that miR-24-3p regulates autophagy by targeting ATG4A PMID:25426560
The autophagy regulator ATG4A was found to be essential for the maintenance of a sub-population with cancer stem cell properties and to regulate breast cancer cell tumourigenicity. PMID:24229464
The demonstration that expression of ATG4A in cells increased stem properties provided an indication of its biological function. Hypomethylation of ATG4A and HIST1H2BN in ovarian tumor-initiating cells predicts poor prognosis for ovarian cancer patients. PMID:24256813
no association between SNP rs807185 and the presence of granulomas in Crohn's disease PMID:21122541
statistical analysis of APG4A gene confirmed the characterisation of each protein-coding gene present in human genome Xq22.1 PMID:21297227
Atg4B possessed the broadest spectrum against all substrates, followed by Atg4A for ATG8 substrates PMID:21177865
HsApg4A cleaves the COOH terminus of GATE-16 PMID:12473658
The cysteine protease HsAtg4 is identified as a direct target for oxidation by hydrogen peroxide, and a cysteine residue located near the HsAtg4 catalytic site as critical for this regulation. PMID:17347651