ATE1 Antibody

Code: CSB-PA002268LA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, IHC, IF

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Target Background

Product Details

Uniprot NO.

O95260

Target Names

ATE1

Alternative Names

AI225793 antibody; Arginine tRNA protein transferase 1 antibody; Arginine-tRNA--protein transferase 1 antibody; Arginyl-tRNA--protein transferase 1 antibody; Arginyl-tRNA-protein transferase antibody; Arginyltransferase 1 antibody; Ate1 antibody; ATE1_HUMAN antibody; AW547406 antibody; EC 2.3.2.8 antibody; MGC26724 antibody; OTTHUMP00000020637 antibody; OTTHUMP00000058665 antibody; R transferase 1 antibody; R-transferase 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Arginyl-tRNA--protein transferase 1 protein (105-253AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-PA002268LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-PA002268LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Target Background

Function(From Uniprot)

Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.

Gene References into Functions

Ate1 is downregulated in several types of human cancer samples at the protein level, and that its transcription level inversely correlates with metastatic progression and patient survival. PMID:26686093
Data indicate that arginyltransferase 1 (ATE1) function controls Myocardin-related transcription factor A (MRTF-A) activity. PMID:25381249

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Subcellular Location

[Isoform ATE1-1]: Nucleus. Cytoplasm.; [Isoform ATE1-2]: Cytoplasm.

Protein Families

R-transferase family

Database Links

HGNC: 782

UNIGENE: Hs.632080

KEGG: hsa:11101

STRING: 9606.ENSP00000224652

OMIM: 607103

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