Abbreviation
Recombinant Paguma larvata ACE2 protein, partial (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD (CSB-MP348663HQE) at 2 μg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml.②Recombinant Paguma larvata ACE2 protein (CSB-MP684964PAL) enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Mca-Ala-Pro-Lys(Dnp)-OH), The Km is 22.84 μM.
Alternative Names
ACE-related carboxypeptidase
Molecular Characterization
Species
Paguma larvata (Masked palm civet)
Expression Region
18-740aa
Target Protein Sequence
QSTTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLFRSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal hFc1-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD (CSB-MP348663HQE) at 2 μg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml.
-
Activity
Recombinant Paguma larvata ACE2 protein (CSB-MP684964PAL) enzyme activity is Activity
Measured by its ability to cleave fluorogenic peptide substrate(Mca-Ala-Pro-Lys(Dnp)-OH), The Km is 22.84 μM.
-
The purity of ACE2 was greater than 90% as determined by SEC-HPLC
Description
ACE2 is a type I membrane protein that functions as the primary receptor for SARS-CoV-2 spike protein, catalyzing the conversion of angiotensin II to angiotensin-(1-7) and playing a critical role in cardiovascular and immune regulation, thus precise characterization of its binding and enzymatic activity is essential for viral entry studies and therapeutic development. This recombinant Paguma larvata ACE2 covers the extracellular region from amino acid 18 to 740 and is produced in mammalian cells with a C-terminal hFc1 tag, preserving native conformation and post-translational modifications necessary for functional integrity. Functional ELISA demonstrates that immobilized SARS-CoV S-RBD (CSB-MP348663HQE) at 2 μg/ml binds Paguma larvata ACE2 with an EC50 of 5.056–7.559 ng/ml, confirming specific receptor-ligand interaction; enzyme activity is measured via cleavage of the fluorogenic peptide substrate Mca-Ala-Pro-Lys(Dnp)-OH, yielding a Km of 22.84 μM. Purity exceeds 90% as determined by both SDS-PAGE and SEC-HPLC, and endotoxin levels are below 1.0 EU/μg, satisfying stringent requirements for cell-based assays and structural biology applications. This active, highly pure, and endotoxin-controlled reagent supports use in viral entry inhibition studies, receptor-binding kinetics, competitive binding assays, and antibody epitope mapping targeting Paguma larvata ACE2.
