Abbreviation
Recombinant Mouse Il2 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Il2ra (CSB-MP011649MO1) at 2 μg/mL can bind Mouse Il2. The EC50 is 95.00-116.2 ng/mL.
Alternative Names
Interleukin-2; IL-2; T-cell growth factor (TCGF); Il2; Il-2
Species
Mus musculus (Mouse)
Expression Region
21-169aa
Target Protein Sequence
APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal hFc1-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Il2ra (CSB-MP011649MO1) at 2 μg/ml can bind Mouse Il2. The EC50 is 95.00-116.2 ng/mL.
Description
Interleukin-2 drives T-cell proliferation and effector differentiation, making it a central regulator in adaptive immunity and a key target in cancer immunotherapy research. This recombinant mouse IL-2, spanning the complete mature protein sequence (aa 21–169), demonstrates specific receptor engagement with an EC50 of 95.00–116.2 ng/mL when binding immobilized IL-2Rα in functional ELISA, providing quantitative evidence that supports its use in T-cell proliferation assays with primary splenocytes or CTLL-2 cells, JAK/STAT3 signaling pathway studies, and as a positive control in cytokine detection platforms. Mammalian expression preserves native post-translational modifications critical for receptor binding, while endotoxin levels below 1.0 EU/μg prevent LPS-driven artifacts that confound interpretation of immune cell activation and cytokine secretion experiments. Purity exceeding 95% by SDS-PAGE, combined with the validated receptor-binding activity, aligns with standards expected in antibody development workflows where IL-2 serves as immunogen or coating antigen.
