Abbreviation
Recombinant Mouse Il2 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0x106 IU/mg.
Alternative Names
Il2; Il-2Interleukin-2; IL-2; T-cell growth factor; TCGF
Species
Mus musculus (Mouse)
Expression Region
21-169aa
Target Protein Sequence
APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Interleukin-2 is a central driver of T cell proliferation and homeostasis, making its recombinant form essential for dissecting JAK/STAT5 signaling, regulatory T cell differentiation, and lymphocyte expansion protocols. This tag-free mouse IL-2, encompassing the full mature sequence (residues 21–169), demonstrates an ED50 below 0.2 ng/ml in CTLL-2 proliferation assays—corresponding to a specific activity exceeding 5.0 × 10⁶ IU/mg—which provides a suitable basis for dose-sensitive functional studies, including primary T cell activation assays, in vivo tumor immunology models, and use as a reference standard in cytokine detection platforms. Endotoxin levels held below 1.0 EU/μg by LAL testing minimize LPS-driven artifacts that can confound immune cell readouts, while purity exceeding 95% by SDS-PAGE supports use in antibody validation workflows such as ELISA standard curves and Western blot positive controls. The E. coli-derived, untagged format ensures that observed biological responses are attributable to IL-2 signaling rather than tag-mediated interference.
