Abbreviation
Recombinant Mouse Fgfr2 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse FGFR2 at 2 μg/mL can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC50 is 2.084-2.249 ng/mL.
Alternative Names
FGFR-2; Keratinocyte growth factor receptor (KGFR); CD332; Fgfr2; Bek, Ect1
Species
Mus musculus (Mouse)
Expression Region
39-263aa
Target Protein Sequence
PYWTNTEKMEKRLHACPAANTVKFRCPAGGNPTSTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCLVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKNGPDGLPYLKVLKHSGINSSNAEVLALFNVTEMDAGEYICKVSNYIGQANQSAWLTVLPKQQAPVREKEITASPDYLE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform Short
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse FGFR2 at 2 μg/ml can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC50 is 2.084-2.249 ng/mL.
Description
FGFR2 mediates critical signaling in development and tissue homeostasis, and its dysregulation drives oncogenesis in multiple epithelial cancers, making receptor-ligand interaction studies essential for therapeutic target validation. This mammalian-expressed construct spanning residues 39–263 of the short isoform captures the extracellular ligand-binding domain with native post-translational modifications that preserve conformational epitopes, as demonstrated by its specific binding to an anti-FGFR2 recombinant antibody with an EC50 of 2.084–2.249 ng/mL in functional ELISA. The quantified binding kinetics support its use in competitive inhibition assays for blocking antibody screening, therapeutic antibody epitope mapping, and small-molecule inhibitor discovery campaigns targeting the FGFR2 extracellular interface. Purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg meet the quality thresholds commonly required for surface plasmon resonance affinity characterization and biolayer interferometry studies where low background interference is critical.
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