Abbreviation
Recombinant Mouse Crtam protein, partial (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Cadm1(CSB-MP004425MO) at 2 μg/mL can bind Mouse Crtam, the EC50 is 54.36 -61.77 ng/mL.
Alternative Names
Class-I MHC-restricted T-cell-associated molecule;CD355
Species
Mus musculus (Mouse)
Expression Region
17-289aa
Target Protein Sequence
AFLKMETVTVEEGQTLTLTCVTSQTKNVSLQWLAPSGFTIFLNQHPALKSSKYQLLHHSATQLSISVSNVTLREEGVYTCLHYGSSVKTKQVRVTVLVTPFQPTVEALVLRRQNGEKSVVLKCSTERSKPPPQITWLLGEGLEIYGELNHEFEADGKICNTSSMLIARAYGKNSTVHCIIQHEGLHGRKLVAPFQFEDLVADQETSDQETSDAPEQSSLSSQALQQPTSTVSMMENSSIPETDKEEKEHATQDPGLSTEASAQHTGLARRKSG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal hFc1-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Cadm1(CSB-MP004425MO) at 2μg/mL can bind Mouse Crtam，the EC50 is 54.36 -61.77 ng/mL.
Description
Crtam regulates cytotoxic T-cell and NK cell effector functions through its interaction with the nectin-like molecule Cadm1, making it a valuable target for dissecting immune synapse formation and lymphocyte-mediated cytotoxicity. This mammalian-expressed construct spanning residues 17–289 demonstrates quantifiable binding to immobilized mouse Cadm1 with an EC50 of 54.36–61.77 ng/mL in functional ELISA, providing a validated reagent for ligand-receptor interaction studies, competitive inhibition assays to screen blocking antibodies, and SPR or BLI experiments characterizing Crtam-Cadm1 affinity under varied conditions. The mammalian expression system supports native glycosylation and disulfide bond formation critical for preserving the immunoglobulin-like domain structure required for Cadm1 recognition. With purity exceeding 90% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this protein meets the quality thresholds commonly required for therapeutic antibody epitope mapping and serves as a positive control in binding assays investigating T-cell co-stimulatory pathways.
