Abbreviation
Recombinant Rhesus macaque MAPT protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta MAPT at 2 μg/mL can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU),the EC50 is 2.464-3.979 ng/mL.
Alternative Names
(Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)
Molecular Characterization
Species
Macaca mulatta (Rhesus macaque)
Expression Region
2-459aa
Target Protein Sequence
AEPRQEFDVMEDHAGTYGLGDRKDQEGYTMLQDQEGDTDAGLKESPLQTPAEDGSEELGSETSDAKSTPTAEDVTAPLVDERAPGEQAAAQPHMEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSATKQVQRKPPPAEPTSERGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPAREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta MAPT at 2 μg/ml can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU),the EC50 is 2.464-3.979 ng/mL.
Description
Tau protein plays a central role in neurodegenerative tauopathies, and this rhesus macaque MAPT provides a translationally relevant ortholog for primate-focused neuroscience research. Produced in a mammalian expression system, this full-length mature protein (aa 2–459) carries an N-terminal 10×His tag and demonstrates confirmed binding activity in a functional ELISA, where immobilized MAPT at 2 μg/mL engaged an anti-MAPT recombinant antibody with an EC₅₀ of 2.464–3.979 ng/mL — supporting direct use in antibody characterization, epitope mapping, and sandwich ELISA development. The endotoxin level below 1.0 EU/μg, combined with greater than 95% purity by SDS-PAGE, satisfies the quality criteria typical for cell-based tau aggregation assays and functional studies examining microtubule binding dynamics. This protein provides a suitable basis for researchers developing cross-species reactive antibodies or investigating tau pathology in non-human primate models of Alzheimer's disease.
