Abbreviation
Recombinant Cynomolgus monkey PTK7 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis PTK7 at 2 μg/mL can bind Anti-PTK7 recombinant antibody (CSB-RA622651MA1HU). The EC50 is 1.399 - 1.666 ng/mL.
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
39-712aa
Target Protein Sequence
AIVFIKQPSSQDALQGRRALLRCEVEAPGLVHVYWLLDGAPVQDTERRFTQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNNTVSSKERNLTLRPAGPEHSGLYSCCAHNAFGQACSSQNFTLSIADESFARVVLAPQDVIVARNEEAMFHCQFSAQPPPNLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPVILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLASIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLKKPQDSQLEEGKPGYLHCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINNVEVYDGTWYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis PTK7 at 2 μg/ml can bind Anti-PTK7 recombinant antibody (CSB-RA622651MA1HU). The EC50 is 1.399 - 1.666 ng/mL.
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The purity of PTK7 was greater than 95% as determined by SEC-HPLC
Description
PTK7 functions as a pseudokinase receptor implicated in Wnt signaling and cell polarity, making it a target of interest in developmental biology and oncology research. This cynomolgus macaque ortholog, spanning residues 39–712 and expressed in mammalian cells to preserve native glycosylation and disulfide architecture, demonstrates quantifiable antibody-binding activity with an EC50 of 1.399–1.666 ng/mL in functional ELISA, confirming that the extracellular domain retains conformational epitopes required for immunological recognition. The validated binding profile supports its use in therapeutic antibody epitope mapping, competitive inhibition screening to identify blocking candidates, and as a positive control in ELISA or surface plasmon resonance assays characterizing anti-PTK7 affinity reagents. Purity exceeding 95% by SDS-PAGE and endotoxin below 1.0 EU/μg align with standards expected in antibody development workflows and receptor-ligand interaction studies where low contaminant levels are critical for reproducible kinetic measurements.
