Abbreviation
Recombinant Cynomolgus monkey CLDN18.2 protein-VLPs (Active)
Purity
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 μg/ml can bind Anti-CLDN18.2 recombinant Antibody (CSB-RA005498A1HU), the EC50 is 2.806-3.641 ng/ml.
Molecular Characterization
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
1-261aa
Target Protein Sequence
MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKTYDGGAHTEDELQSYPSKHDYV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged (This tag can be tested only under denaturing conditions)
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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CSB-MP4304MOV is detected by Mouse anti-6*His monoclonal antibody.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 μg/ml can bind Anti-CLDN18.2 recombinant Antibody (CSB-RA005498A1HU), the EC50 is 2.806-3.641 ng/ml.
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The purity of VLPs was greater than 90% as determined by SEC-HPLC
Description
Claudin 18.2 has emerged as a high-priority therapeutic target in oncology due to its restricted expression pattern and surface accessibility on tumor cells, yet its four-transmembrane topology makes soluble recombinant production notoriously difficult. This Macaca fascicularis CLDN18.2 construct (full-length, aa 1–261) overcomes that challenge by presenting the protein in virus-like particles (VLPs) derived from mammalian cells, preserving native conformation and extracellular loop architecture critical for antibody epitope recognition. Functional ELISA confirms robust binding to an anti-CLDN18.2 recombinant antibody (CSB-RA005498A1HU) with an EC50 of 2.806–3.641 ng/ml, directly supporting antibody development, cross-reactivity screening against cynomolgus orthologs, and validation of therapeutic candidates targeting CLDN18.2 in tumor metastasis research. The VLP format, combined with endotoxin levels below 1.0 EU/μg, provides a suitable basis for immunization protocols and cell-based binding assays where conformational integrity is essential.
