Abbreviation
Recombinant Cynomolgus monkey CD44 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/mL can bind Anti-CD44 monoclonal antibody (CSB-MA004938A0m), the EC50 is 64.18-90.91 ng/mL.
Alternative Names
(Extracellular matrix receptor III)(GP90 lymphocyte homing/adhesion receptor)(HUTCH-I)(Hermes antigen)(Hyaluronate receptor)(Phagocytic glycoprotein 1)(Phagocytic glycoprotein I)
Molecular Characterization
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
21-650aa
Target Protein Sequence
QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSVGFETCRYGFIEGHVVIPRIQPNSICAANHTGVYILTSNTSQYDTYCFNASAPPKEDCTSVTDLPNAFDGPITITIVNPDGTRYIKKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFHTFSTAHPIPDEDGPWITDSTDRIPATTLMSTSATATETATKRQETQDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRVFDHTKQNKDWTQWNPSHSNPEVLLQTTARITADVDRNGTTAHEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQAIPSSTTEETATQKEQWFGNRWHEGYLQTPREDSHSTTGTAAASALTSHPMQGRTTPSPEDSSWTDFFDPISHPMGRGHQAGRRMDMDSSHSTTLQPTADPNTGLVEDLDRTGPLSMTTKQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPDTKESRTFIPVTSAKTGSFGVTEVTVGDSNSNVNHSLSGDQDAFYPSGGSHTTHGSESAGHSHGSQEGGANTTSGPVRTPQIPE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/ml can bind Anti-CD44 monoclonal antibody (CSB-MA004938A0m), the EC50 is 64.18-90.91 ng/mL.
Description
CD44 serves as a critical adhesion molecule mediating hyaluronate binding, cell migration, and tumor metastasis signaling, making it a high-value target in oncology and immune cell trafficking research. This cynomolgus monkey CD44 construct (aa 21–650) encompasses the extracellular and transmembrane domains, providing broad functional domain coverage suitable for antibody development, cell adhesion and spreading assays, and integrin-ligand binding studies. Mammalian cell expression preserves native glycosylation and folding, and validated binding activity confirms proper conformation: immobilized at 2 μg/mL, the protein binds an anti-CD44 monoclonal antibody with an EC50 of 64.18–90.91 ng/mL in functional ELISA, supporting use in antibody screening and cross-reactivity validation for preclinical primate models. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this C-terminal His-tagged protein satisfies the quality criteria typical for cell migration and invasion assays as well as tumor metastasis research.
