Abbreviation
Recombinant Cynomolgus monkey ALPP protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Activity
Unit Definition:One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate(pNPP), in 1 minute at 37°C, pH10.0.The specific activity is >7000 U/mg.
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
21-504aa
Target Protein Sequence
IIPVEEENPDFWNRQAAEALGAAKKLQPIQTAAKNLIIFLGDGMGVSTVTAARILKGQKEEKLGPETPLAMDHFPYVALSKTYSVDKHVPDSAATATAYLCGVKGNFQTIGLSAAARYNQCNTTRGNEVVSVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDANMPGSARREGCKDIATQLISNMDIDVILGGGRKYMFRMGAPDPEYPHDYSQDGTRMDGKNLVQEWLAKHQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGAYPLRGSSIFGLAPGKAQDRKAYTALLYGNGPGYVLKDGARPDVTESESGSPEYRQQAAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPAGTTD
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
Placental alkaline phosphatase serves as a biomarker in several malignancies and plays critical roles in phosphate metabolism during development, making the cynomolgus macaque ortholog valuable for translational studies bridging rodent models and human biology. This full-length mature protein (aa 21–504) demonstrates robust catalytic function with a specific activity exceeding 7000 U/mg against p-nitrophenylphosphate at pH 10.0 and 37°C, providing the quantitative performance required for kinetic parameter analysis including Km and Vmax determination, as well as inhibitor screening with accurate IC50 measurements. The C-terminal 10xHis tag preserves active site accessibility while enabling straightforward purification, and the >95% purity confirmed by SDS-PAGE supports its use as a positive control in enzyme-linked assays and for substrate specificity profiling where contaminating phosphatases would confound results. Endotoxin levels below 1.0 EU/μg align with standards expected in cell-based inhibition assays and drug candidate evaluation studies.
