Abbreviation
Recombinant Human Wnt3a surrogate protein (Active)
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T cells. The ED50 for this effect is 17.76-26.50 ng/mL.
Target Names
Wnt3a surrogate
Species
Homo sapiens (Human)
Target Protein Sequence
N/A
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal hFc1-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered 25 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.2
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its ability to induce Topflash reporter activity in HEK293T cells. The ED50 for this effect is 17.76-26.50 ng/mL.
Description
Wnt3a surrogate proteins address the challenge of producing native Wnt3a, which is notoriously difficult to express in active form due to extensive post-translational lipidation requirements. This full-length construct (aa 1–306) demonstrates robust canonical Wnt signaling activity in HEK293T cells, inducing Topflash reporter responses with an ED50 of 17.76–26.50 ng/mL—a potency range that supports dose-response studies in stem cell differentiation protocols and mechanistic investigations of β-catenin-dependent transcription. Mammalian expression ensures native-like glycosylation and folding, critical for receptor engagement in assays probing Frizzled–LRP5/6 interactions or screening small-molecule Wnt pathway modulators. The protein meets quality thresholds commonly required for cell-based functional assays, with purity exceeding 90% by SDS-PAGE and endotoxin below 1.0 EU/μg, making it appropriate for neuronal differentiation models, osteogenic lineage commitment studies, and receptor-ligand competition experiments where low background and consistent activity are essential.
