Abbreviation
Recombinant Human SHH protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of >1.0x103 IU/mg.
Alternative Names
HHG 1; HHG-1; HHG1; HLP 3; HLP3; Holoprosencephaly 3; HPE 3; HPE3; MCOPCB5; shh; SHH_HUMAN; SMMC I; SMMCI; Sonic Hedgehog (Drosophila) homolog; sonic hedgehog homolog (Drosophila); Sonic hedgehog homolog; Sonic hedgehog protein; Sonic hedgehog protein C-product; TPT; TPTPS
Species
Homo sapiens (Human)
Expression Region
22-197aa
Target Protein Sequence
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial(22-24:LAC→IVI)
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
Sonic hedgehog signaling drives critical patterning events in development and is frequently dysregulated in cancers such as basal cell carcinoma and medulloblastoma, making precise pathway reconstitution essential for tumor biology research. This tag-free recombinant human SHH fragment (residues 25–197) encompasses the biologically active N-terminal signaling domain and demonstrates an ED50 below 1 μg/ml in a C3H/10T1/2 alkaline phosphatase induction assay, corresponding to a specific activity exceeding 1.0 × 10³ IU/mg—performance that supports osteogenic differentiation studies, stem cell fate commitment experiments, and receptor-ligand binding assays using SPR or ELISA formats. The E. coli expression system yields a non-glycosylated protein that provides a defined baseline for dissecting glycosylation-independent Patched-1 binding interactions. Purity exceeding 98% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based proliferation assays and in vivo tumor biology models requiring minimal innate immune interference.
