Abbreviation
Recombinant Human SELPLG protein, partial (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human SELPLG at 2 μg/mL can bind Anti-SELPLG recombinant antibody (CSB-RA621767MA1HU). The EC50 is 2.420-2.733 ng/mL.
Alternative Names
P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD antigen CD162
Species
Homo sapiens (Human)
Expression Region
18-320aa
Target Protein Sequence
LQLWDTWADEAEKALGPLLARDRRQATEYEYLDYDFLPETEPPEMLRNSTDTTPLTGPGTPESTTVEPAARRSTGLDAGGAVTELTTELANMGNLSTDSAAMEIQTTQPAATEAQTTQPVPTEAQTTPLAATEAQTTRLTATEAQTTPLAATEAQTTPPAATEAQTTQPTGLEAQTTAPAAMEAQTTAPAAMEAQTTPPAAMEAQTTQTTAMEAQTTAPEATEAQTTQPTATEAQTTPLAAMEALSTEPSATEALSMEPTTKRGLFIPFSVSSVTHKGIPMAASNLSVNYPVGAPDHISVKQC
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human SELPLG at 2 μg/ml can bind Anti-SELPLG recombinant antibody (CSB-RA621767MA1HU). The EC50 is 2.420-2.733 ng/mL.
Description
PSGL-1 mediates leukocyte rolling and tethering to activated endothelium through high-affinity interactions with P-selectin, a process central to immune surveillance and cancer metastasis. This recombinant fragment spanning residues 18–320 captures the functionally critical N-terminal region and demonstrates robust binding activity in functional ELISA, achieving an EC50 of 2.420–2.733 ng/mL against anti-SELPLG antibody when immobilized at 2 μg/mL—quantitative evidence that supports its use in selectin-ligand binding studies and antibody development workflows. Mammalian expression preserves the native glycosylation and post-translational modifications essential for physiologically relevant selectin recognition, enabling researchers to model leukocyte adhesion dynamics in cell migration assays and investigate PSGL-1's role in tumor metastasis. The protein meets quality thresholds commonly required for functional assays, with purity exceeding 90% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, criteria that align with standards expected in cell-based adhesion and invasion experiments.
