Abbreviation
Recombinant Human NTMT1 protein (Active)
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Activity
Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 μg/ml can bind human NTMT1, the EC50 of human NTMT1 protein is 3.029-5.404 μg/ml.
Research Area
Cell Biology
Alternative Names
Alpha N-terminal protein methyltransferase 1A;Methyltransferase-like protein 11A;N-terminal RCC1 methyltransferase;X-Pro-Lys N-terminal protein methyltransferase 1A;NTM1A
Species
Homo sapiens (Human)
Expression Region
2-223aa
Target Protein Sequence
TSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal GST-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 μg/ml can bind human NTMT1, the EC50 of human NTMT1 protein is 3.029-5.404 μg/ml.
Description
NTMT1 catalyzes the trimethylation of N-terminal α-amines on X-Pro-Lys motifs, a modification implicated in DNA damage repair and mitotic chromosome segregation. This recombinant human NTMT1 covers the full-length mature protein (aa 2–223) and carries an N-terminal GST tag, positioning the fusion partner away from the catalytic domain to preserve active-site accessibility for substrate engagement. Functional validation confirms binding activity against immobilized CCL5 (2 μg/ml) with an EC50 of 3.029–5.404 μg/ml, supporting its use as a positive control in enzyme-linked binding assays and providing a suitable basis for inhibitor screening and IC50 determination. Purity exceeding 85% by SDS-PAGE meets the quality thresholds commonly required for enzymatic activity assays, substrate specificity profiling, and kinetic parameter analysis including Km and kcat measurements.
