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Recombinant Human Lysosome-associated membrane glycoprotein 2 (LAMP2), partial (Active)

Recombinant Human Lysosome-associated membrane glycoprotein 2 (LAMP2), partial (Active)

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Code: CSB-AP005471HU

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10ug50ug500ug1mg(1mg*1 or 500ug*2)

US$154

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Express system: Mammalian cell

Species: Homo sapiens (Human)

Tag Info: C-terminal 6xHis-tagged

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Target Background

Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

The ED50 as determined by its ability to bind Human LGALS-3 in functional ELISA is less than 30 ug/ml.

Target Names

LAMP2

Uniprot NO.

P13473

Research Area

Cancer

Alternative Names

LAMP2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LGP-96; CD antigen CD107b

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

29-375aa

Target Protein Sequence

LELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.94 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.2

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage Condition

Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Lead Time

Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Shelf Life

The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.

Troubleshooting and FAQs

Protein FAQs

Datasheet & COA

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Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Description

Recombinant Human LAMP2 is a partial lysosome-associated membrane glycoprotein 2 (CD107b) that features the 29-375aa expression region. This protein is an essential tool for cancer research and is obtained from mammalian cells with a C-terminal 6xHis-tag. With a purity of greater than 95% as determined by SDS-PAGE, LAMP2 has an endotoxin level of less than 1.0 EU/µg as assessed by the LAL method. The functional activity of LAMP2 is demonstrated by its capacity to bind human LGALS-3 in a functional ELISA, with an ED₅₀ of less than 30 µg/ml. This lyophilized powder format enables easy storage and application across various research projects.

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Target Background

Function(From Uniprot)

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation. Plays a role in lysosomal protein degradation in response to starvation. Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens.; Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.; (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Gene References into Functions

Knockdown of key autophagy proteins in combination with sunitinib showed similar effect as chloroquine. Sunitinib also induced lysosomal membrane permeabilization, which further increased in the presence of chloroquine or knockdown of lysosome-associated membrane protein (LAMP2). Both combinations led to cell death PMID:28729403
Results demonstrated that LAMP2 expression levels correlated with tumor histological differentiation and TNM stages. PMID:28453465
Since effective regimens are readily available timely psychiatric evaluation is warranted in all newly diagnosed subjects with LAMP2 mutations, regardless of whether they show the typical Danon disease medical (cardiac) symptoms or not. PMID:28627787
identified LAMP-2 as an endocytic receptor on monocyte-derived dendritic cells (MoDC) that routes cargo into unusual Ag processing pathways, which reduces surface expression of Ag-derived peptides while selectively enriching Ag within immunogenic exosome; this novel pathway has implications for the initiation of immune responses both locally and at distant sites PMID:28607115
The results provide a new insight that LAMP-2 contributes to the ROS clearance and cell death induced by Zn(2+) treatment, which would help us to get a better understanding of Zn(2+)-induced toxicity in respiratory system. PMID:28483530
overexpression assists neuroendocrine differentiation of prostate cancer cells induced by serum deprivation and facilitates autophagy activity PMID:27627761
Genetic analysis identified 2 novel LAMP2 gene mutations. In Family A, a G-A transition (c.962G > A) leading to a nonsense mutation at codon 321 (p.Trp321Ter), and in Family B, a one-nucleotide insertion (c.973insC) leading to a full frame-shift (p.Pro324+24X) was detected in exon 8 of the LAMP2 gene. PMID:27179547
intracellular Salmonella recruit the host proteins LAMP-2A and Hsc73, key components of the host protein turnover pathway known as chaperone-mediated autophagy involved in transport of cytosolic proteins to the lysosome for degradation. PMID:27932462
3 novel nonsense mutations (p.Q240X, p.S250X, and p.G22X) were found in LAMP2 associated with early onset Danon disease with hypertrophic cardiomyopathy. LAMP2 expression was absent in both cardiac and skeletal muscle samples of the first proband and severely decreased LAMP2 expression in the skeletal muscle samples of the second proband. PMID:27460667
Collectively, the present study shows that impaired Lamp2a expression in hepatocellular carcinoma contributes to tumor cell viability and promotes tumor growth and recurrence. PMID:27840904
Increased expression of LAMP2 has been observed in peripheral blood mononuclear cells of coronary artery disease patients compared to the control group. PMID:27923262
Knockdown of LAMP2A, a CMA-related protein, and TSG101, an mA-related protein, significantly but only partially decreased the punctate accumulation of GAPDH-HT in AD293 cells and primary cultured rat cortical neurons. PMID:27377049
miR-487b-5p regulates temozolomide resistance of lung cancer cells through LAMP2-mediated autophagy. PMID:27097129
Up-regulation of LAMP2 is associated with carcinogenesis and progression of Salivary Adenoid Cystic Carcinoma. PMID:26350055
In our study of the EOG in Danon disease, we show for the first time to our knowledge that a LAMP2 mutation may cause a primary retinal pigment epitheliopathy. PMID:26398689
LAMP-2C serves as a natural inhibitor of chaperone-mediated autophagy that can selectively skew MHCII presentation of cytoplasmic antigens PMID:26856698
In the early stages of Parkinson's disease, with LAMP2A selectively reduced in association with increased alpha-synuclein, and decreased levels of heat shock cognate protein 70. PMID:25594542
This study showed that LAMP2 upregulation occurs in vitro and in vivo in neoplastic cells. PMID:26658462
Down-regulation of LAMP2A expression could inhibit cell proliferation in multiple myeloma cells PMID:25940285
Data show thart lysosome-associated membrane protein type 2a (LAMP-2A) forms a coiled coil helix trimer in n-dodecylphosphocholine micelle, and protein substrates interact with its cytosolic tail. PMID:25342746
LAMP2 has a role in differentiation of primary biliary cirrhosis PMID:24007661
Patient B harbored a frame-shift deletion mutation in exon 3 (c.396delA) leading to a truncated LAMP2 protein PMID:24691104
down-regulation of LAMP2A could reduce the resistance of breast cancer cells to paclitaxel PMID:24721399
LAMP2 is investigated as a marker of Epstein-Barr virus-mediated B lymphocyte transformation in lysosomal storage diseases. PMID:24068328
Autoantibodies to hLAMP-2 that bind native glomerular but not neutrophil hLAMP-2 are found in patients with ANCA-negative pauci-immune focal necrotizing glomerulonephritis. PMID:24203998
These data support a positive relationship between anti-LAMP-2 antibody and cutaneous vasculitis. PMID:23704322
Decreased levels of the chaperone-mediated autophagy proteins LAMP-2A and hsc70 (CMA) in Parkinson's disease brain samples suggests compromised alpha-synuclein degradation by CMA and may underpin the Lewy body pathology. PMID:23492776
indicate that monoclonal antibodies specific to CD107a (LAMP-1) or CD107b (LAMP-2) enhanced LPS-induced IL-8 secretion of THP-1 cells. PMID:23603048
expression of LAMP2A was observed in breast tumor tissues of all patients under investigation, suggesting a survival mechanism via chaperone-mediated autophagy and LAMP2A. PMID:22874552
Studies suggest that Hsc70 and lysosome-associated protein 2A (LAMP-2A) through chaperone-mediated autophagy (CMA) play a role in the clearance of Htt and suggest a novel strategy to target the degradation of mutant huntingtin (Htt). PMID:23071649
There is a progressive, age-related decrease of LAMP-2 gene expression in the peripheral leukocytes of healthy subjects, indicating a trend of decreasing autophagy activities with aging. PMID:22732524
variants within LAMP-2 gene promoter may be linked to Parkinson disease. PMID:22867958
findings indicated that patients with Danon disease caused by mutations in exon 1 - 8 manifested as a typically severe phenotype, while patients with mutations in exon 9 of the LAMP2B isoform presented with a relatively benign phenotype PMID:22541782
A novel LAMP2 mutation (c.940delG) in Danon disease patients, which results in a putatively truncated protein. PMID:22365987
decreased LAMP-2 gene expression and increased LC3 gene expression may contribute to the pathogenesis of sporadic Parkinson's disease PMID:21514572
Peripheral leukocyte LAMP-2 expression is significantly inceased in coronary artery disease. PMID:21462217
intrafamilial phenotypic variability in Danon disease is related to a novel LAMP-2 mutation PMID:21161685
gene deficient B cells exhibit altered MHC class II presentation of exogenous antigens PMID:20518820
Lysosome-associated membrane protein (LAMP2) cardiomyopathy is an X-linked and highly progressive myocardial storage disorder associated with diminished survival, which clinically resembles sarcomeric hypertrophic cardiomyopathy. PMID:20920663
The LAMP2 microdeletion mechanism appears to involve 1 Alu-mediated unequal recombination and 2 chromosomal breakage points involving TA-rich repeat sequences. PMID:20173215
Danon disease is caused by deficiency of lysosome-associated membrane protein-2 (LAMP-2). PMID:20513107
Data show that the BCG phagosome is relatively depleted in LAMP-2, NPC1, flotillin-1, vATPase, and syntaxin 3. PMID:19815536
This studu showed decreased LAMP2 expression in the sketal muscle in female patient with Danon disease. PMID:14561493
The glycogen-storage cardiomyopathy produced by LAMP2 or PRKAG2 mutations resembles hypertrophic cardiomyopathy but is distinguished by electrophysiological abnormalities PMID:15673802
role for the lysosomal Lamp-2a-hsc70 complex in promoting immunological recognition and antigen presentation PMID:15894275
LAMP2 mutations may account for significant proportion of cases of hypertrophic cardiomyopathy children, especially when skeletal myopathy and/or Wolff-Parkinson-White syndrome is present. Danon disease may be underrecognized in pediatric cardiology. PMID:16144992
Our report further expands the phenotype of Danon disease by describing retinopathy in 3 cases. A thorough clinical examination, including ophthalmic investigation, is needed in all cases of Danon disease. PMID:17296900
The biopsied muscle specimen stained for LAMP2 and confirmed the diagnosis of vacuolar myopathy with dilated cardiomyopathy. PMID:17873513
Analysis of the lysosome-associated membrane protein-2 (LAMP-2) gene detected a novel mutation, confirming a diagnosis of Danon disease. PMID:17899313
A new intronic mutation in the LAMP2 gene in French Candian family leading to out frame skppin of exon 7 in Dannon disease. PMID:18004770

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, autophagosome membrane. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Protein Families

LAMP family

Tissue Specificity

Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung

Database Links

HGNC: 6501

UNIGENE: Hs.496684

KEGG: hsa:3920

OMIM: 300257

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