Abbreviation
Recombinant Human CSF2RB protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CSF2RB at 2 μg/mL can bind anti-CSF2RB antibody（CSB-RA006047A0HU）, the EC50 is 0.1594-0.3046 ng/mL.
Alternative Names
(CDw131)(GM-CSF/IL-3/IL-5 receptor common beta subunit)(CD antigen CD131)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
17-443aa
Target Protein Sequence
WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized CSF2RB at 2 μg/ml can bind anti-CSF2RB antibody（CSB-RA006047A0HU）, the EC50 is 0.1594-0.3046 ng/mL.
Description
CSF2RB serves as the shared signaling subunit for the IL-3, IL-5, and GM-CSF receptors, making it a critical node in myeloid and eosinophil biology research. This recombinant human CSF2RB encompasses the extracellular domain (aa 17–443) produced in mammalian cells to preserve native glycosylation and conformational integrity essential for accurate ligand-receptor interaction studies. Functional ELISA confirms binding activity with an EC50 of 0.1594–0.3046 ng/mL against an anti-CSF2RB antibody, supporting use in receptor-ligand interaction assays, blocking antibody screening, therapeutic antibody epitope mapping, and as a positive control in SPR or BLI affinity characterization experiments. The C-terminal 6×His tag facilitates oriented immobilization, while purity exceeding 95% and endotoxin levels below 1.0 EU/μg satisfy the criteria typically required for sensitive binding assays and drug discovery screens targeting cytokine signaling pathways.
