Abbreviation
Recombinant Dog MS4A1 protein-VLPs (Active)
Purity
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Dog CD20 at 10 μg/mL can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC50 is 147.3-199.0 ng/mL.
Alternative Names
(Membrane-spanning 4-domains subfamily A member 1)(CD antigen CD20)
Molecular Characterization
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region
1-297aa
Target Protein Sequence
MTTPRNSMSGTLPVDPMKSPTAMYPVQKIIPKRMPSVVGPTQNFFMRESKTLGAVQIMNGLFHIALGSLLMIHTDVCAPICITMWYPLWGGIMFIISGSLLAAADKNPRKSLVKGKMIMNSLSLFAAISGIIFLIMDIFNITISHFFKMENLNLIKAPMPYVDIHNCDPANPSEKNSLSIQYCGSIRSVFLGVFAVMLIFAFFQKLVTAGIVENEWKKLCSKPKSDVVVLLAAEEKKEQPIETTEEMVELTEIASQPKKEEDIEIIPVQEEEGELEINFAEPPQEQESSPIENDSIP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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CSB-MP661636DO is detected by Mouse anti-6*His monoclonal antibody.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Dog CD20 at 10 μg/ml can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC50 is 147.3-199.0 ng/mL.
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The purity of VLPs was greater than 90% as determined by SEC-HPLC
Description
CD20 presents a significant challenge as a multi-pass transmembrane target, and this virus-like particle (VLP) format preserves the native conformation of full-length canine CD20 (aa 1–297) by displaying it within a membrane context—critical for generating conformationally relevant immune responses and accurate binding data. Functional ELISA confirms that immobilized dog CD20-VLPs bind an anti-CD20 recombinant antibody with an EC50 of 147.3–199.0 ng/mL, validating the protein's suitability as an antigen for therapeutic antibody screening, cross-reactivity assessment, and immunization campaigns targeting canine lymphoma. The mammalian expression system ensures proper folding and post-translational modifications, while endotoxin levels below 1.0 EU/μg satisfy criteria typical for cell-based functional assays and in vivo immunization studies in veterinary oncology models. The C-terminal 10×His tag, detectable under denaturing conditions, supports use in Western blot-based quality control without interfering with native-conformation binding assays.
