Abbreviation
Recombinant Cricetulus griseus HSPA5 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Cricetulus griseus HSPA5 at 2 μg/mL can bind Anti-HSPA5 recombinant antibody (CSB-RA010827MA1HU). The EC50 is 1.884-2.205 ng/mL.
Alternative Names
Endoplasmic reticulum chaperone BiP; EC:3.6.4.10; 8 kDa glucose-regulated protein; Binding-immunoglobulin protein; Heat shock protein 70 family protein 5; Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein ; HSPA5; GRP78; I79_019946
Species
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Expression Region
19-654aa
Target Protein Sequence
EEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTSEKDEL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Cricetulus griseus HSPA5 at 2 μg/ml can bind Anti-HSPA5 recombinant antibody (CSB-RA010827MA1HU). The EC50 is 1.884-2.205 ng/mL.
Description
BiP (HSPA5) orchestrates protein folding quality control in the endoplasmic reticulum and translocates to the cell surface in stressed or malignant cells, making it a pivotal target in cancer biology and unfolded protein response research. This full-length mature protein (aa 19–654) demonstrates quantifiable binding activity with an EC50 of 1.884–2.205 ng/mL against anti-HSPA5 antibody in functional ELISA, providing a validated reagent for antibody-antigen interaction studies, inhibitor screening campaigns, and IC50 determination assays targeting BiP-client protein interfaces. The C-terminal 10×His tag preserves the N-terminal ATPase domain and substrate-binding pocket, supporting use in enzymatic activity assays that measure ATP hydrolysis rates and in kinetic parameter analysis to define Km and kcat values for chaperone-substrate interactions. Endotoxin levels below 1.0 EU/μg and purity exceeding 95% meet the stringent criteria required for cell-based assays investigating ER stress pathways and for serving as a positive control in enzyme-linked functional screens.
